Advanced bacterial polyhydroxyalkanoates: Towards a versatile and sustainable platform for unnatural tailor-made polyesters SCIE SCOPUS

Cited 109 time in WEB OF SCIENCE Cited 121 time in Scopus
Title
Advanced bacterial polyhydroxyalkanoates: Towards a versatile and sustainable platform for unnatural tailor-made polyesters
Author(s)
Park, Si Jae; Kim, Tae Wan; Kim, Min Kyung; Lee, Sang Yup; Lim, Sung-Chul
Publication Year
2012-11
Abstract
Polyhydroxyalkanoates (PHAs) are biopolyesters that generally consist of 3-, 4-, 5-, and 6-hydroxycarboxylic acids, which are accumulated as carbon and energy storage materials in many bacteria in limited growth conditions with excess carbon sources. Due to the diverse substrate specificities of PHA synthases, the key enzymes for PHA biosynthesis, PHAs with different material properties have been synthesized by incorporating different monomer components with differing compositions. Also, engineering PHA synthases using in vitro-directed evolution and site-directed mutagenesis facilitates the synthesis of PHA copolymers with novel material properties by broadening the spectrum of monomers available for PHA biosynthesis. Based on the understanding of metabolism of PHA biosynthesis, recombinant bacteria have been engineered to produce different types of PHAs by expressing heterologous PHA biosynthesis genes, and by creating and enhancing the metabolic pathways to efficiently generate precursors for PHA monomers. Recently, the PHA biosynthesis system has been expanded to produce unnatural biopolyesters containing 2-hydroxyacid monomers such as glycolate, lactate, and 2-hydroxybutyrate by employing natural and engineered PHA synthases. Using this system, polylactic acid (PLA), one of the major commercially-available bioplastics, can be synthesized from renewable resources by direct fermentation of recombinant bacteria. In this review, we discuss recent advances in the development of the PHA biosynthesis system as a platform for tailor-made polyesters with novel material properties. (c) 2011 Elsevier Inc. All rights reserved.
ISSN
0734-9750
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3429
DOI
10.1016/j.biotechadv.2011.11.007
Bibliographic Citation
BIOTECHNOLOGY ADVANCES, v.30, no.6, pp.1196 - 1206, 2012
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Subject
RECOMBINANT ESCHERICHIA-COLI; PROPIONATE COA-TRANSFERASE; LA-BASED POLYESTERS; IN-VITRO EVOLUTION; POLYLACTIC ACID; PHA SYNTHASE; RALSTONIA-EUTROPHA; SATURATION MUTAGENESIS; BIOSYNTHESIS GENES; MOLECULAR-WEIGHT
Keywords
PHA; PLA; PHA synthase; PLA copolymer; 2-hydroxyacid containing PHA
Type
Article
Language
English
Document Type
Review
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
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