Assessment of EvaGreen-based quantitative real-time PCR assay for enumeration of the microalgae Heterosigma and Chattonella (Raphidophyceae) SCIE SCOPUS

DC Field Value Language
dc.contributor.author Park, Bum Soo -
dc.contributor.author Baek, Seung Ho -
dc.contributor.author Ki, Jang-Seu -
dc.contributor.author Cattolico, Rose Ann -
dc.contributor.author Han, Myung-Soo -
dc.date.accessioned 2020-04-20T06:40:19Z -
dc.date.available 2020-04-20T06:40:19Z -
dc.date.created 2020-01-28 -
dc.date.issued 2012-12 -
dc.identifier.issn 0921-8971 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/3420 -
dc.description.abstract Heterosigma akashiwo and Chattonella species (Raphidophyceae) are difficult to detect and quantify in environmental samples because of their pleomorphic and fragile cell nature. In this study, we developed a quantitative real-time polymerase chain reaction (qRT-PCR) assay for the enumeration of these algal taxa using a new DNA-binding dye, EvaGreen. Species-specific qRT PCR primers to H. akashiwo, Chattonella antiqua, Chattonella marina, Chattonella ovata, and Chattonella subsalsa were designed to target the ITS2 rRNA gene intergenic region. Primer specificities were tested via BLAST searches. In addition, specificity was verified using empirical tests, including competitive PCR. The qRT PCR assay analyzing C (t) value and the log of cell number showed a significant linear relationship (r (2) a parts per thousand yenaEuro parts per thousand 0.997). When light microscopy was used to monitor the population dynamics of targeted Raphidophyceae from Lake Shihwa, H. akashiwo was detected in ten samples and no Chattonella spp. were detected (70 samples collected from May, 2007 to January, 2008). In contrast, when the qRT-PCR assay was used, H. akashiwo was detected in 41 samples. C. antiqua, C. marina, and C. ovata were detected in eight samples. Most of the samples analyzed using qRT-PCR assays showed higher algal numbers than did those assayed via microscopy, suggesting that the enumeration of Raphidophyceae via classic microscopic methods most likely underestimates true algal concentration. -
dc.description.uri 1 -
dc.language English -
dc.publisher SPRINGER -
dc.subject POLYMERASE-CHAIN-REACTION -
dc.subject RIBOSOMAL-RNA GENE -
dc.subject SYBR-GREEN-I -
dc.subject PARASITE PERKINSUS-MARINUS -
dc.subject MELTING CURVE ANALYSIS -
dc.subject RED TIDE ALGAE -
dc.subject PFIESTERIA-PISCICIDA -
dc.subject ENVIRONMENTAL-SAMPLES -
dc.subject RAPID DETECTION -
dc.subject DNA FRAGMENTS -
dc.title Assessment of EvaGreen-based quantitative real-time PCR assay for enumeration of the microalgae Heterosigma and Chattonella (Raphidophyceae) -
dc.type Article -
dc.citation.endPage 1567 -
dc.citation.startPage 1555 -
dc.citation.title JOURNAL OF APPLIED PHYCOLOGY -
dc.citation.volume 24 -
dc.citation.number 6 -
dc.contributor.alternativeName 백승호 -
dc.identifier.bibliographicCitation JOURNAL OF APPLIED PHYCOLOGY, v.24, no.6, pp.1555 - 1567 -
dc.identifier.doi 10.1007/s10811-012-9816-2 -
dc.identifier.scopusid 2-s2.0-84867860527 -
dc.identifier.wosid 000310232900023 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus POLYMERASE-CHAIN-REACTION -
dc.subject.keywordPlus RIBOSOMAL-RNA GENE -
dc.subject.keywordPlus SYBR-GREEN-I -
dc.subject.keywordPlus PARASITE PERKINSUS-MARINUS -
dc.subject.keywordPlus MELTING CURVE ANALYSIS -
dc.subject.keywordPlus RED TIDE ALGAE -
dc.subject.keywordPlus PFIESTERIA-PISCICIDA -
dc.subject.keywordPlus ENVIRONMENTAL-SAMPLES -
dc.subject.keywordPlus RAPID DETECTION -
dc.subject.keywordPlus DNA FRAGMENTS -
dc.subject.keywordAuthor Raphidophyceae -
dc.subject.keywordAuthor Quantitative real-time PCR -
dc.subject.keywordAuthor EvaGreen -
dc.subject.keywordAuthor Field application -
dc.relation.journalWebOfScienceCategory Biotechnology & Applied Microbiology -
dc.relation.journalWebOfScienceCategory Marine & Freshwater Biology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Biotechnology & Applied Microbiology -
dc.relation.journalResearchArea Marine & Freshwater Biology -
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