Antimicrobial Gageomacrolactins Characterized from the Fermentation of the Marine-Derived Bacterium Bacillus subtilis under Optimum Growth Conditions SCIE SCOPUS

Cited 30 time in WEB OF SCIENCE Cited 35 time in Scopus
Title
Antimicrobial Gageomacrolactins Characterized from the Fermentation of the Marine-Derived Bacterium Bacillus subtilis under Optimum Growth Conditions
Author(s)
Tareq, Fakir Shahidullah; Kim, Ji Hye; Lee, Min Ah; Lee, Hyi-Seung; Lee, Jong-Seok; Lee, Yeon-Ju; Shin, Hee Jae
KIOST Author(s)
Lee, Min Ah(이민아)Lee, Hyi Seung(이희승)Lee, Jong Seok(이종석)Lee, Yeon Ju(이연주)Shin, Hee Jae(신희재)
Alternative Author(s)
TAREQ; 김지혜; 이민아; 이희승; 이종석; 이연주; 신희재
Publication Year
2013-04-10
Abstract
Marine bacteria are a potential source of structurally diversified bioactive secondary metabolites that are not found in terrestrial sources. In our continuous effort to search for new antimicrobial agents from marine-derived bacteria, we isolated bacterial strain 109GGC020 from a marine sediment sample collected from Gageocho, Republic of Korea. The strain was identified as Bacillus subtilis based on a 16s rRNA sequence analysis. After a 7-day fermentation of the B. subtilis strain under optimum growth conditions three new and four known secondary metabolites were discovered using chromatographic procedures, and their biological activities were evaluated against both bacteria and crop-devastating fungi. The discovered metabolites were confirmed by extensive 2D NMR and high-resolution ESI-MS data analyses to have the structures of new macrolactin derivatives gageomacrolactins 1-3 and known macrolactins A (4), B (5), F (6), and W (7). The stereoconfigurations of 1-3 were assigned based on coupling constant values, chemical derivatization studies, and a literature review. The coupling constants were very crucial to determine the relative geometries of olefins in 1-3 because of overlap of the H-1 NMR signals. The NMR data of these compounds were recorded in different solvents to overcome this problem and obtain accurate coupling constant values. The new macrolactin derivatives 1-3 displayed good antibiotic properties against both Gram-positive (S. aureus, B. subtilis, and B. cereus) and Gram-negative (E. coli, S. typhi, and P. aeruginosa) bacteria with minimum inhibitory concentration (MIC) values of 0.02-0.05 mu M. Additionally, the antifungal activities of 1-7 were evaluated against pathogenic fungi and found to inhibit mycelial growth of A. niger, B. cinerea, C. acutatum, C. albicans, and R. solani with MIC values of 0.04-0.3 mu M, demonstrating that these compounds were good fungicides.
ISSN
0021-8561
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3238
DOI
10.1021/jf4009229
Bibliographic Citation
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.61, no.14, pp.3428 - 3434, 2013
Publisher
AMER CHEMICAL SOC
Subject
ANTIBACTERIAL; MACROLACTINS
Keywords
B. subtilis; marine sediment; antibacterial activity; fungicides; stereoconfigurations
Type
Article
Language
English
Document Type
Article
Publisher
AMER CHEMICAL SOC
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