Immune response-related gene expression profile of a novel molluscan I kappa B protein member from Manila clam (Ruditapes philippinarum) SCIE SCOPUS

DC Field Value Language
dc.contributor.author Lee, Youngdeuk -
dc.contributor.author Wickamarachchi, Wickramaarachchige Don Niroshana -
dc.contributor.author Whang, Ilson -
dc.contributor.author Oh, Minyoung -
dc.contributor.author Umasuthan, Navaneethaiyer -
dc.contributor.author Zoysa, Mahanama De -
dc.contributor.author Oh, Chulhong -
dc.contributor.author Kang, Do-Hyung -
dc.contributor.author Lee, Jehee -
dc.date.accessioned 2020-04-20T05:55:53Z -
dc.date.available 2020-04-20T05:55:53Z -
dc.date.created 2020-02-18 -
dc.date.issued 2013-02 -
dc.identifier.issn 0301-4851 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/3229 -
dc.description.abstract Mollusks lack an adaptive immune system and rely solely on the innate immune response. The nuclear factor-kappa B (NF-kappa B) signaling pathway is one of the most important components of the innate immune system and its activity is regulated by physical interaction with the inhibitor of NF-kappa B (I kappa B) protein. The manila clam, Ruditapes philippinarum (Rp), is a key species of the world's aquaculture industry, and recent pathogenic threats, such as the Gram-negative lipopolysaccharide (LPS)-expressing Vibrio tapetis bacteria, have produced severe adverse economic impacts. Here, we describe identification, characterization and immune responses of novel I kappa B (Rp-I kappa B) in the manila clam. The Rp-I kappa B cDNA is comprised of a 1,032 bp open reading frame, which encodes 343 amino acid residues and has a predicted molecular mass of 38 kDa. The Rp-I kappa B protein exhibits typical structural features of I kappa B family members, including the I kappa B degradation motif, PEST sequence, and six ankyrin repeats. Phylogenetic analysis showed that manila clam and other known molluscan I kappa B proteins grouped together in the invertebrate cluster. Analysis of the tissue expression distribution revealed that Rp-I kappa B was ubiquitously expressed. However, immune challenge with V. tapetis and purified LPS endotoxin induced significant up-regulation of Rp-I kappa B expression in gill and hemocytes. These results indicated that Rp-I kappa B may play an important role in manila clam defense against bacterial infection. -
dc.description.uri 1 -
dc.language English -
dc.publisher SPRINGER -
dc.subject BROWN RING DISEASE -
dc.subject VIBRIO-TAPETIS -
dc.subject DEFENSE PARAMETERS -
dc.subject ALPHA-INHIBITOR -
dc.subject PACIFIC OYSTER -
dc.subject PHOSPHORYLATION -
dc.subject CLONING -
dc.subject ACTIVATION -
dc.subject SEQUENCE -
dc.subject PATHWAY -
dc.title Immune response-related gene expression profile of a novel molluscan I kappa B protein member from Manila clam (Ruditapes philippinarum) -
dc.type Article -
dc.citation.endPage 1527 -
dc.citation.startPage 1519 -
dc.citation.title MOLECULAR BIOLOGY REPORTS -
dc.citation.volume 40 -
dc.citation.number 2 -
dc.contributor.alternativeName 이영득 -
dc.contributor.alternativeName 오철홍 -
dc.contributor.alternativeName 강도형 -
dc.identifier.bibliographicCitation MOLECULAR BIOLOGY REPORTS, v.40, no.2, pp.1519 - 1527 -
dc.identifier.doi 10.1007/s11033-012-2196-5 -
dc.identifier.scopusid 2-s2.0-84877616743 -
dc.identifier.wosid 000313165500084 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus BROWN RING DISEASE -
dc.subject.keywordPlus VIBRIO-TAPETIS -
dc.subject.keywordPlus DEFENSE PARAMETERS -
dc.subject.keywordPlus ALPHA-INHIBITOR -
dc.subject.keywordPlus PACIFIC OYSTER -
dc.subject.keywordPlus PHOSPHORYLATION -
dc.subject.keywordPlus CLONING -
dc.subject.keywordPlus ACTIVATION -
dc.subject.keywordPlus SEQUENCE -
dc.subject.keywordPlus PATHWAY -
dc.subject.keywordAuthor Manila clams -
dc.subject.keywordAuthor Ruditapes philippinarum -
dc.subject.keywordAuthor IkappaB, mollusk -
dc.subject.keywordAuthor Quantitative real time RT-PCR -
dc.relation.journalWebOfScienceCategory Biochemistry & Molecular Biology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Biochemistry & Molecular Biology -
Appears in Collections:
Jeju Research Institute > Jeju Bio Research Center > 1. Journal Articles
Jeju Research Institute > Tropical & Subtropical Research Center > 1. Journal Articles
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