Immune response-related gene expression profile of a novel molluscan I kappa B protein member from Manila clam (Ruditapes philippinarum) SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Lee, Youngdeuk | - |
dc.contributor.author | Wickamarachchi, Wickramaarachchige Don Niroshana | - |
dc.contributor.author | Whang, Ilson | - |
dc.contributor.author | Oh, Minyoung | - |
dc.contributor.author | Umasuthan, Navaneethaiyer | - |
dc.contributor.author | Zoysa, Mahanama De | - |
dc.contributor.author | Oh, Chulhong | - |
dc.contributor.author | Kang, Do-Hyung | - |
dc.contributor.author | Lee, Jehee | - |
dc.date.accessioned | 2020-04-20T05:55:53Z | - |
dc.date.available | 2020-04-20T05:55:53Z | - |
dc.date.created | 2020-02-18 | - |
dc.date.issued | 2013-02 | - |
dc.identifier.issn | 0301-4851 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/3229 | - |
dc.description.abstract | Mollusks lack an adaptive immune system and rely solely on the innate immune response. The nuclear factor-kappa B (NF-kappa B) signaling pathway is one of the most important components of the innate immune system and its activity is regulated by physical interaction with the inhibitor of NF-kappa B (I kappa B) protein. The manila clam, Ruditapes philippinarum (Rp), is a key species of the world's aquaculture industry, and recent pathogenic threats, such as the Gram-negative lipopolysaccharide (LPS)-expressing Vibrio tapetis bacteria, have produced severe adverse economic impacts. Here, we describe identification, characterization and immune responses of novel I kappa B (Rp-I kappa B) in the manila clam. The Rp-I kappa B cDNA is comprised of a 1,032 bp open reading frame, which encodes 343 amino acid residues and has a predicted molecular mass of 38 kDa. The Rp-I kappa B protein exhibits typical structural features of I kappa B family members, including the I kappa B degradation motif, PEST sequence, and six ankyrin repeats. Phylogenetic analysis showed that manila clam and other known molluscan I kappa B proteins grouped together in the invertebrate cluster. Analysis of the tissue expression distribution revealed that Rp-I kappa B was ubiquitously expressed. However, immune challenge with V. tapetis and purified LPS endotoxin induced significant up-regulation of Rp-I kappa B expression in gill and hemocytes. These results indicated that Rp-I kappa B may play an important role in manila clam defense against bacterial infection. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.subject | BROWN RING DISEASE | - |
dc.subject | VIBRIO-TAPETIS | - |
dc.subject | DEFENSE PARAMETERS | - |
dc.subject | ALPHA-INHIBITOR | - |
dc.subject | PACIFIC OYSTER | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | CLONING | - |
dc.subject | ACTIVATION | - |
dc.subject | SEQUENCE | - |
dc.subject | PATHWAY | - |
dc.title | Immune response-related gene expression profile of a novel molluscan I kappa B protein member from Manila clam (Ruditapes philippinarum) | - |
dc.type | Article | - |
dc.citation.endPage | 1527 | - |
dc.citation.startPage | 1519 | - |
dc.citation.title | MOLECULAR BIOLOGY REPORTS | - |
dc.citation.volume | 40 | - |
dc.citation.number | 2 | - |
dc.contributor.alternativeName | 이영득 | - |
dc.contributor.alternativeName | 오철홍 | - |
dc.contributor.alternativeName | 강도형 | - |
dc.identifier.bibliographicCitation | MOLECULAR BIOLOGY REPORTS, v.40, no.2, pp.1519 - 1527 | - |
dc.identifier.doi | 10.1007/s11033-012-2196-5 | - |
dc.identifier.scopusid | 2-s2.0-84877616743 | - |
dc.identifier.wosid | 000313165500084 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | BROWN RING DISEASE | - |
dc.subject.keywordPlus | VIBRIO-TAPETIS | - |
dc.subject.keywordPlus | DEFENSE PARAMETERS | - |
dc.subject.keywordPlus | ALPHA-INHIBITOR | - |
dc.subject.keywordPlus | PACIFIC OYSTER | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordAuthor | Manila clams | - |
dc.subject.keywordAuthor | Ruditapes philippinarum | - |
dc.subject.keywordAuthor | IkappaB, mollusk | - |
dc.subject.keywordAuthor | Quantitative real time RT-PCR | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |