Immune response-related gene expression profile of a novel molluscan I kappa B protein member from Manila clam (Ruditapes philippinarum) SCIE SCOPUS

Cited 22 time in WEB OF SCIENCE Cited 24 time in Scopus
Title
Immune response-related gene expression profile of a novel molluscan I kappa B protein member from Manila clam (Ruditapes philippinarum)
Author(s)
Lee, Youngdeuk; Wickamarachchi, Wickramaarachchige Don Niroshana; Whang, Ilson; Oh, Minyoung; Umasuthan, Navaneethaiyer; Zoysa, Mahanama De; Oh, Chulhong; Kang, Do-Hyung; Lee, Jehee
KIOST Author(s)
Lee, Young Deuk(이영득)Oh, Chul Hong(오철홍)Kang, Do Hyung(강도형)
Publication Year
2013-02
Abstract
Mollusks lack an adaptive immune system and rely solely on the innate immune response. The nuclear factor-kappa B (NF-kappa B) signaling pathway is one of the most important components of the innate immune system and its activity is regulated by physical interaction with the inhibitor of NF-kappa B (I kappa B) protein. The manila clam, Ruditapes philippinarum (Rp), is a key species of the world's aquaculture industry, and recent pathogenic threats, such as the Gram-negative lipopolysaccharide (LPS)-expressing Vibrio tapetis bacteria, have produced severe adverse economic impacts. Here, we describe identification, characterization and immune responses of novel I kappa B (Rp-I kappa B) in the manila clam. The Rp-I kappa B cDNA is comprised of a 1,032 bp open reading frame, which encodes 343 amino acid residues and has a predicted molecular mass of 38 kDa. The Rp-I kappa B protein exhibits typical structural features of I kappa B family members, including the I kappa B degradation motif, PEST sequence, and six ankyrin repeats. Phylogenetic analysis showed that manila clam and other known molluscan I kappa B proteins grouped together in the invertebrate cluster. Analysis of the tissue expression distribution revealed that Rp-I kappa B was ubiquitously expressed. However, immune challenge with V. tapetis and purified LPS endotoxin induced significant up-regulation of Rp-I kappa B expression in gill and hemocytes. These results indicated that Rp-I kappa B may play an important role in manila clam defense against bacterial infection.
ISSN
0301-4851
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3229
DOI
10.1007/s11033-012-2196-5
Bibliographic Citation
MOLECULAR BIOLOGY REPORTS, v.40, no.2, pp.1519 - 1527, 2013
Publisher
SPRINGER
Subject
BROWN RING DISEASE; VIBRIO-TAPETIS; DEFENSE PARAMETERS; ALPHA-INHIBITOR; PACIFIC OYSTER; PHOSPHORYLATION; CLONING; ACTIVATION; SEQUENCE; PATHWAY
Keywords
Manila clams; Ruditapes philippinarum; IkappaB, mollusk; Quantitative real time RT-PCR
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
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