Peridinin chlorophyll protein 유전자의 특성규명

Peridinin chlorophyll protein 유전자의 특성규명
Alternative Title
Characterization of the major light harvesting protein, peridinin chlorophyll protein gene of Alexandrium tamarense
진언선; 김현진; 이택견; 장만
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
Photosynthetic dinoflagellates contain a membrane bound light harvesting complex similar to that of higher plants. In addition, they have developed a water-soluble antenna, peridinin chlorophyll protein (PCP), which has no sequence similarity with other known proteins. Although extensive studies have examined PCP proteins, only a few complete pcp nucleotide sequences have been reported to date. PCPs occur in two different forms, a short form with a molecular mass of 15 kDa and a long form with a mass of 32-35 kDa. This study describes PCP and its cDNA from Alexandrium tamarence. mRNA isolated from Alexandrium tamarence was used for the construction of cDNA libraries. A cDNA clone was identified which encoded the PCP including a 55 amino acid transit peptide and the 314 amino acid mature protein. The deduced amino acid sequence clearly contains an internal duplication, implying that amongst dinoflagellates the long form of PCP has arisen by duplication and fusion of genes encoding short form. The predicted amino acid sequence displayed a 50%-70% identity with the other known pcp sequences. Expression and regulation of this PCP gene will be discussed.
Bibliographic Citation
7th International congress of Plant Molecular Biology, pp.07 - 20, 2003
Barcellona, Spain
Barcellona, Spain
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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