Evaluation of biomarker potential of cytochrome P450 1A (CYP1A) gene in the marine medaka, Oryzias melastigma exposed to water-accommodated fractions (WAFs) of Iranian crude oil
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Title
- Evaluation of biomarker potential of cytochrome P450 1A (CYP1A) gene in the marine medaka, Oryzias melastigma exposed to water-accommodated fractions (WAFs) of Iranian crude oil
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Author(s)
- Kim, Ryeo-Ok; Kim, Bo-Mi; Hwang, Dae-Sik; Au, Doris W. T.; Jung, Jee-Hyun; Shim, Won Joon; Leung, Kenneth M. Y.; Wu, Rudolf S. S.; Rhee, Jae-Sung; Lee, Jae-Seong
- KIOST Author(s)
- Jung, Jee Hyun(정지현); Shim, Won Joon(심원준)
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Alternative Author(s)
- 정지현; 심원준
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Publication Year
- 2013-03
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Abstract
- CYP1A is involved in the metabolism of diverse chemicals, including polycyclic aromatic hydrocarbons and alkylated-PAHs, as a first line of detoxification mechanism. First, we identified and characterized the CYP1A gene from the marine medaka, Oryzias melastigma. O. melastigma CYP1A (Om-CYP1A) showed a high similarity of motifs/domains compared to those of vertebrates in their amino acid sequences. To check whether the Om-CYP1A would be inducible, we tested two strong CYP1A inducers, beta-naphthoflavone (beta-NF) and benzo[alpha] pyrene (B[alpha]P), and observed concentration-dependent transient expression on transcripts of Om-CYP1A for 96 h over a wide range of concentrations. Om-CYP1A mRNA level was significantly increased in exposure to different concentrations of beta-NF and B[alpha]P, and its expression was highly transcribed within 12 h upon the exposure to low concentrations of both chemicals. Inducible transcript profiles revealed that Om-CYP1A would be associated with the toxicant metabolism via AhREs/DREs/XREs in its promoter region. To uncover the effects of the water-accommodated fraction (WAF) of crude oil on transcripts of Om-CYP1A, we measured mRNA expression of Om-CYP1A towards different concentrations of WAF for 24 h. As a result, WAF exposure significantly increased Om-CYP1A transcripts at all concentrations as well as during time-course experiments for 96 h. In this paper, we demonstrated that WAF would trigger up-regulation of the CYP1A gene that would be associated with the initiation of the cellular defense systems. This finding provides a better understanding of the molecular mechanism of cellular protection particularly that involved in the WAF-mediated cellular response in O. melastigma. (c) 2012 Elsevier Inc. All rights reserved.
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ISSN
- 1532-0456
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/3220
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DOI
- 10.1016/j.cbpc.2012.11.003
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Bibliographic Citation
- COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, v.157, no.2, pp.172 - 182, 2013
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Publisher
- ELSEVIER SCIENCE INC
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Subject
- POLYCYCLIC AROMATIC-HYDROCARBONS; FUNDULUS-HETEROCLITUS; EROD ACTIVITY; XENOBIOTIC BIOTRANSFORMATION; MOLECULAR-CLONING; MESSENGER-RNA; IN-VIVO; EXPRESSION; INDUCTION; FISH
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Keywords
- CYP1A; Marine medaka; Oryzias melastigma; Water-accommodated fraction; WAF
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Type
- Article
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Language
- English
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Document Type
- Article
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