TBT와 Sea Nine-211이 북방대합 해독효소계에 미치는 영향

Abstract

Sea-Nine 211 (4,5-dichloro-2-n-octyl-3(2H) isothiazolone) was injected to clam, Pseudocardium sachalinensis, that inhabitate nothern part of Kangwon Province, Korea. Survival rate of the clam and xenobiotics metabolizing enzyme activities in digestive gland were measured during the 4 day-exposure period. The results were compared with those of TBT exposed clam. There were no mortality of clam in the solvent (DMSO) control group and the three Sea-Nine 211 exposure groups (5, 25, 50 mg/kg body weight), while the clam exposed to 1, 2, 5 mg/kg TBT chloride (TBTC) demonstrated 70, 30, 0% survival rate, respectively. The Sea-Nine 211 exposure group showed a tendency of CYP450 induction according to the exposure duration, on the other hand, CYP450 content was decreased in the TBT exposure group. NADPH cytochrome P450 reductase activity slightly increased according to the exposure duration in the Sea-Nine 211 exposure group, while TBTC inhibit its activity as CYP450 content. There was no significant change of cytochrome b5 reductase activity in the clam exposed to Sea-Nine 211. In the TBTC exposure group, its activity increased in early exposure period and then significantly decreased the rest of exposure period. CYP1A1 dependant EROD activities in both the Sea-Nine 211 and the TBTC exposure groups showed no significant differences compared to those of the solvent control group. CYP1A2 dependant MROD activity was significantly induced at the end of exposure, but no significant difference was obtained in the TBTC exposure group. CYP2B1/2 dependant PROD activities in both the Sea-Nine 211 and the TBTC exposure groups showed no significant differences compared to those of the solvent control group. GST activity in the clam exposed to Sea-Nine 211 demonstrated less than those of the solvent control group in the early exposure period, and then recovered at the end of exposure, while its activity was significantly different at the end of exposure in the TBTC exposure