해양미생물유래 유화능이 우수한 신규 세포외다당류의 개발

Title
해양미생물유래 유화능이 우수한 신규 세포외다당류의 개발
Alternative Title
Development of novel marine microbial exopolysaccharide with high emulsifying ability
Author(s)
이홍금
Publication Year
2004-04-16
Abstract
A marine bacterial strain, Alteromonas sp.00SS11568 was isolated from biofilm samples collected in coastal regions of South Sea, Korea. This strain produced exopolysaccharide, designated as p-11568, which exhibited emulsifying ability. The p-11568 was found to consist of glucose and galactose as main natural sugars with the molar ratio of these sugars being 1.3:1 with uronic acid (18.9%, w/w) and sulfate groups (1.2% w/w) approximately. The average molecular mass was 4.4 x 105 daltons by gel filtration chromatography. Emulsification Index (E24) of 0.1% p-11568 was 77.4% with emulsified kerosene content and higher than those of commercial polysaccharides such as xanthan gum (26.1%), gellan gum (1.3%) and sodium alginate (2.0%), respectively. The intrinsic viscosity of p-11568 was 12.9 g/dl and 12.5 g/dl by Huggins and Karemer equation and the overlap of p-11568 was 0.29 g/dl. p-11568 conformation was similar with caboxymethylcellulose on the degree of rigidness (5.9 x 10-2). Rheological behavior of p-11568 dispersions indicates the formation of an intermediate structure between a random-coil polysaccharide and a week gel. Aqueous dispersions of p-11568 at the concentrations ranging from 0.1 % to 1.0 % (w/v) showed marked shear-thinning properties as Power-law behavior. In aqueous dispersions of 1.0% p-11568, consistency index (K) and flow behavior index (n) were 4,404 and 0.42, respectively. The p-11568 was stable to pH, salts (NaCl and CaCl2) and temperature.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/31961
Bibliographic Citation
생물공학의 동향(XIV), pp.31, 2004
Publisher
한국생물공학회
Type
Conference
Language
English
Publisher
한국생물공학회
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