해면 공생 방선균의 분리

Abstract

We isolated symbiotic actinomycetes from marine sponges Hymeniacidon perleve, and identified them using phylogenetic analysis of 16S rDNA sequences. Sponges (Porifera) have been recognized as a rich source of novel bioactive compounds. Sponges contain symbiotic microorganisms that have been suggested to be associated with biosynthesis of the novel compounds. Actinomycetes also have been regarded as a large library bank of novel bioactive compounds. Therefore, symbiotic actinomycetes isolated from marine sponges are strong candidates for new source of novel compound. We collected marine sponges Hymeniacidon perleve, and treated them with chemical and physical methods. We used 13 different kinds of media to culture marine actinomycetes, and total 110 actinomycetes strains were isolated. When we added the marine sponge extracts to media, the number of bacteria increased. But there was no significant change in actinomycetes number. The corresponding maximum number was 3.6×102 cfu per 1 g of wet sponge samples. The phylogenetic analysis of the actinomycetes showed that there were several new species candidates.