남극 및 한국연안에 서식하는 방선균의 분리

Abstract

We isolated symbiotic marine actinomycetes from sponges, and soil actinomycetes from Antarctic region. Actinomycetes strains were succeeding cultured on ISP4 agar plates at 25℃, and 74 strains were finally isolated. The isolated actinomycetes were inoculated into Bennett liquid media, genomic DNA was extracted, and phylogenetic analysis was performed on the basis of 16S rDNA sequences. Streptomyces diastaticus and S. intermedius were isolated from a sponge. S. diastaticus isolated in this study showed circle form and white color colonies, and they produced substrate mycelium. S. intermedius is known as a plant pathogenic bacteria. S. intermedius isolated in this study showed concentric circle form and white color colonies, and they produced substrate mycelium. Streptomyces caviscabies was isolated from Antarctic soil. Type species of S. caviscabies was isolated from potatoes, and this is first report on appearance of S. caviscabies in Antarctic region. S. caviscabies isolated in this study showed circle form and white-green color colonies with clear zone, and they produced substrate mycelium.