Molecular Cloning, Overexpression, and Enzymatic Characterization of Glycosyl Hydrolase Family 16 beta-Agarase from Marine Bacterium Saccharophagus sp AG21 in Escherichia coli SCIE SCOPUS KCI
DC Field | Value | Language |
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dc.contributor.author | Youngdeuk, Lee | - |
dc.contributor.author | Oh, Chulhong | - |
dc.contributor.author | De Zoysa, Mahanarna | - |
dc.contributor.author | Kim, Hyowon | - |
dc.contributor.author | Wickramaarachchi, Wickramaarachchige Don Niroshana | - |
dc.contributor.author | Whang, Ilson | - |
dc.contributor.author | Kang, Do-Hyung | - |
dc.contributor.author | Lee, Jehee | - |
dc.date.accessioned | 2020-04-20T05:40:26Z | - |
dc.date.available | 2020-04-20T05:40:26Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2013-07 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/3156 | - |
dc.description.abstract | An agar-degrading bacterium was isolated from red seaweed (Gelidium amansii) on a natural seawater agar plate, and identified as Saccharophagus sp. AG21. The beta-agarase gene from Saccharophagus sp. AG21 (agy1) was screened by long and accurate (LA)-PCR. The predicted sequence has a 1,908 bp open reading frame encoding 636 amino acids (aa), and includes a glycosyl hydrolase family 16 (GH16) beta-agarase module and two carbohydrate binding modules of family 6 (CBM6). The deduced aa sequence showed 93.7% and 84.9% similarity to beta-agarase of Saccharophagus degradans and Microbulbifer agarilyticus, respectively. The mature agy1 was cloned and overexpressed as a His-tagged recombinant beta-agarase (rAgy1) in Escherichia coli, and had a predicted molecular mass of 69 kDa and an isoelectric point of 4.5. rAgy1 showed optimum activity at 55 degrees C and pH 7.6, and had a specific activity of 85 U/mg. The rAgy1 activity was enhanced by FeSO4 (40%), KCl (34%), and NaCl (34%), compared with the control. The newly identified rAgy1 is a beta-agarase, which acts to degrade agarose to neoagarotetraose (NA4) and neoagarohexaose (NA6) and may be useful for applications in the cosmetics, food, bioethanol, and reagent industries. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | CARBOHYDRATE-BINDING MODULES | - |
dc.subject | BIOCHEMICAL-CHARACTERIZATION | - |
dc.subject | CALCIUM-BINDING | - |
dc.subject | PURIFICATION | - |
dc.subject | AGAROSE | - |
dc.subject | EXPRESSION | - |
dc.subject | XYLANASE | - |
dc.subject | STABILITY | - |
dc.subject | DOMAINS | - |
dc.subject | GENE | - |
dc.title | Molecular Cloning, Overexpression, and Enzymatic Characterization of Glycosyl Hydrolase Family 16 beta-Agarase from Marine Bacterium Saccharophagus sp AG21 in Escherichia coli | - |
dc.type | Article | - |
dc.citation.endPage | 922 | - |
dc.citation.startPage | 913 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 23 | - |
dc.citation.number | 7 | - |
dc.contributor.alternativeName | 이영득 | - |
dc.contributor.alternativeName | 오철홍 | - |
dc.contributor.alternativeName | 강도형 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.23, no.7, pp.913 - 922 | - |
dc.identifier.doi | 10.4014/jmb.1209.09009 | - |
dc.identifier.scopusid | 2-s2.0-84880685511 | - |
dc.identifier.wosid | 000322502800004 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001790088 | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CARBOHYDRATE-BINDING MODULES | - |
dc.subject.keywordPlus | BIOCHEMICAL-CHARACTERIZATION | - |
dc.subject.keywordPlus | CALCIUM-BINDING | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | AGAROSE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | XYLANASE | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | DOMAINS | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordAuthor | Saccharophagus sp AG21 | - |
dc.subject.keywordAuthor | beta-agarase | - |
dc.subject.keywordAuthor | GH16 | - |
dc.subject.keywordAuthor | neoagaro-oligosaccharide | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |