독성 Alexandrium tamarense의 EST 분석 및 삭시톡신 생합성 유전자의 확인 KCI

Title
독성 Alexandrium tamarense의 EST 분석 및 삭시톡신 생합성 유전자의 확인
Alternative Title
Expressed Sequence Tag Analysis of Toxic Alexandrium tamarense and Identification of Saxitoxin Biosynthetic Genes
Author(s)
장만; 이주연; 정영재; 이건섭; 김동균; 이택견
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
2013-07
Abstract
A. tamarense로부터 ESTs library를 제작하였다. 이들의 염기서열을 분석하여 STX 생합성 관련 유전자를 클로닝하였다. 연구결과 827 클론의 염기서열이 분석되었고, 564개의 EST가 GenBank에서의 Blast search를 사용하여 기능에 따라 분류되었다. EST에서의 주요 유전자는 cellular organization, cell metabolism, energy, cell cycle과 DNA processing, cellular transport와 transport, cell rescue, defense, death와 aging 및 transcription 등으로 분석되었다. 특히 S-adenosylmethionine synthetase와 H2A histone family 유전자의 발현이 독성 A. tamarense에서 증가하였다. 이러한 결과는 두 개의 유전자가 A. tamarense에서의 삭시톡신 생합성을 검출하기 위한 좋은 바이오마커가 될 수 있음을 보여준다.

Expressed sequence tag (EST) library was constructed from A. tamarense. Base sequences of EST clones were analyzed and saxitoxin biosynthesis-related genes were cloned. Sequences of 827 clones were analyzed and 564 EST were functionally clustered using Blast searches against GenBank. Main genes in the EST had functions on cellular organization, cell metabolism, energy, cell cycle and DNA processing, cellular transport and transport, cell rescue, defense, death and aging, and transcription. Moreover, expression of S-adenosylmethionine synthetase and H2A histone family genes were increased in the toxic A. tamarense. These results show that two genes could be a good biomarkers for the detection of saxitoxin biosynthesis in the A. tamarense.
ISSN
1975-4701
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3153
DOI
10.5762/KAIS.2013.14.7.3582
Bibliographic Citation
한국산학기술학회논문지, v.14, no.7, pp.3582 - 3588, 2013
Publisher
한국산학기술학회
Keywords
Alexandrium tamarense; Biomarker; Biosynthetic genes; ESTs; Saxitoxin
Type
Article
Language
Korean
Publisher
한국산학기술학회
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse