해양미생물을 이용한 광학특이 Epoxide Hydrolase 탐색

Title
해양미생물을 이용한 광학특이 Epoxide Hydrolase 탐색
Alternative Title
Screening of Enantioselective Epoxide Hydrolase from a Marine Bacterium.
Author(s)
황영옥; 우정희; 강성균; 한명수; 김상진
KIOST Author(s)
Kang, Sung Gyun(강성균)
Publication Year
2005-05-12
Abstract
Enantioselective synthesis or hydrolysis is getting much more attention due to current concerns about mixed chirality of a lot of chemical, pesticides and medicine. To screen strains producing an epoxide hydrolase(EH) which hydrolyzes (R) or (S) - epoxide preferentially, 120 strains isolated from a variety of marine environments primarily by the capability of living on styrene oxide were tested for EH activity using spectrophotometric assay. Among those, one strain (JCS358) was selected by enantioselective hydrolysis of styrene oxide, confirmed by gas chromatography(GC). The EH from JCS358 preferentially hydrolysed the (R)-epoxides of styrene oxide. GC result, showed that enantiopure (S)-epoxides would be obtain with a value of 98% ee(enantiomeric excess).This study presents a first example which discovered an enantioselective epoxide hydrolase from marine environment successfully.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/31483
Bibliographic Citation
한국미생물학회 프로시딩, pp.C006, 2005
Publisher
한국미생물학회
Type
Conference
Language
English
Publisher
한국미생물학회
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