심해퇴적물 메타게놈으로부터 저온 적응된 신규 리파제의 특성
DC Field | Value | Language |
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dc.contributor.author | 전정호 | - |
dc.contributor.author | 임재규 | - |
dc.contributor.author | 배승섭 | - |
dc.contributor.author | 양성현 | - |
dc.contributor.author | 이현숙 | - |
dc.contributor.author | 강성균 | - |
dc.contributor.author | 김상진 | - |
dc.contributor.author | 이정현 | - |
dc.date.accessioned | 2020-07-17T07:52:15Z | - |
dc.date.available | 2020-07-17T07:52:15Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2005-10-09 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/31310 | - |
dc.description.abstract | To obtain novel biocatalysts, 8,823 clones library in Escherichia coli using fosmid with DNA extracted directly from deep-sea sediments had previously been constructed. The isolation of the lipase gene screened on tricaprylin-containing agar plates. Sequence analysis appeared an open reading frame (EML1) consisting of 915 nucleotides that encoded a protein (EML1) of 304 amino acid with a molecular mass 33,600 Da. We performed gene expression and purification of the recombinant protein. The enzyme was stable between pH 8 and 8.5, and the optimal pH for the enzymatic hydrolysis of olive oil was about 8. The optimal temperature for EML1 was 25 ℃, and it was unstable at temperature higher than 40 ℃. The activation energy for the hydrolysis of olive oil was determined to be 3.28 kcal/mol in the temperature range 5 to 30 ℃. The enzyme was increased lipase activity in the presence of Zn2+, Mg2+, Cu2+ and Fe2+ but was inhibited by Ca2+. The addition of detergents enhanced the enzyme activity but containing SDS almost abolished lipase activity. When triglyceride substrates of various chain-lengths were examined, the lipase showed highest activity towards trilaurin (C12). Natural substrates presented high lipase activity for saturated fatty acid such as coconut oil, palm oil. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | 국제효소공학회 | - |
dc.relation.isPartOf | 국제효소공학회(18차) | - |
dc.title | 심해퇴적물 메타게놈으로부터 저온 적응된 신규 리파제의 특성 | - |
dc.title.alternative | Characterization of novel cold-adapted lipase from metagenomic resources of deep sea sediments | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 93 | - |
dc.citation.startPage | 93 | - |
dc.citation.title | 국제효소공학회(18차) | - |
dc.contributor.alternativeName | 전정호 | - |
dc.contributor.alternativeName | 임재규 | - |
dc.contributor.alternativeName | 배승섭 | - |
dc.contributor.alternativeName | 양성현 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.contributor.alternativeName | 김상진 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.identifier.bibliographicCitation | 국제효소공학회(18차), pp.93 | - |
dc.description.journalClass | 1 | - |