해양미생물로부터 광학특이성 epoxide hydrolase 의 탐색 및 특성분석

Title
해양미생물로부터 광학특이성 epoxide hydrolase 의 탐색 및 특성분석
Alternative Title
Screening, cloning and characterization of enantioselective epoxide hydrolase from a marine bacterium
Author(s)
황영옥; 우정희; 강성균; 김상진; 한명수
KIOST Author(s)
Kang, Sung Gyun(강성균)
Publication Year
2005-10-09
Abstract
Enantioselective synthesis or hydrolysis is getting much more attention due to current concerns about mixed chirality of a lot of chemical, pesticides and medicine. To screen strains producing an epoxide hydrolase(EH) which hydrolyzes (R) or (S) - epoxide preferentially, 120 strains isolated from a variety of marine environments primarily by the capability of living on styrene oxide were tested for EH activity using spectrophotometric assay. Among those, one strain(JCS358) was selected by enantioselective hydrolysis of styrene oxide, confirmed by gas chromatography. The enantioselective EH gene was cloned from screening a genomic library of the isolate, and overexpressed heterologously in E. coli using T7 promoter driven vector system. The protein was purified using His-tag affinity chromatography, and the purified protein was further characterized in terms of enantioselective hydrolyzing activity. This study presents a first example which discovered an enantioselective epoxide hydrolase from marine environment successfully.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/31306
Bibliographic Citation
국제효소공학회 프로시딩, pp.98, 2005
Publisher
국제효소공학회
Type
Conference
Language
English
Publisher
국제효소공학회
Related Researcher
Research Interests

marine biotechnology,microbiology,해양생명공학,미생물학

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