세포표면 소수성이 높은 균주가 세균군집의 표면부착에 미치는 영향

Abstract

Cell-cell interactions during the process of biofilm formation are important for the future biofilm architecture. In the present study, short-term interactions between microorganisms has different cell surface properties (hydrophobicity (H) and attachment ability on surface (A)) were investigated using two set of model biofilm communities consist of 4 strains each. Biofilm formation on acrylic surfaces was conducted with 50 ml conical tubes containing aged seawater at room temperature for 3 hours. The amount of microbial cells adhered on surfaces was determined by measuring decrease of optical density of seawater and by measuring the amount of crystal violet stained on surfaces. Strains have high H/A were adhered well on surfaces (over 90%) but the strains have high A does not (10~30%). Adherence of microorganisms on surfaces was increased 10~20% when the strains were mixed. Cultures containing strains have high H/A always showing increased adherence than that of expected. Strains have high H/A might enhances the adherence of other strains on surfaces. Further investigation about the factors concerning on cell-cell interactions is required.