규조류 Phaeodactylum tricornutum으로의 녹색형광단백질 형질전환

Abstract

Diatom was responsible to 75% of O2 and CO2 concentration in earth and commonly used to feeds in mariculture of fishery. Diatom Phaeodactylum tricornutum was well known species in their biological and physiological process than any other species. In recently, the knowledge about them is raising to issue, because of their usefulness on each industry. But their molecular biological approach is still being on infant states. This problem is due to difficult transformation method, the absence of gene information, the diversity of phytoplankton, and so on. We are going to try and error in transformation of Phaeodactylum tricornutum. This study about unicellular eukaryotic phytoplankton has many impacts about plant research, so-called study that related or about evolution of chloroplast. We introduced enhanced green fluorescent protein (EGFP) and sugar transporter1 (STP1) of Arabidopsis thaliana incorporated pPha-T1 vector in Diatom. Plasmid pPha-T1 vector had promoter and terminator of fucoxanthin-chlorophyll a/c binding protein (FcpA) and promoter-terminator expression cassette for heterologous recombination in Phaeodactylum tricornutum. As transformation method, biolistic particle bombardment system was used. We evaluated this study will be a good system for unicellular application of plant and chloroplast with evolutional relationship.