Expressions of oxidative stress-related genes and antioxidant enzyme activities in Mytilus galloprovincialis (Bivalvia, Mollusca) exposed to hypoxia SCIE SCOPUS

Cited 36 time in WEB OF SCIENCE Cited 38 time in Scopus
Title
Expressions of oxidative stress-related genes and antioxidant enzyme activities in Mytilus galloprovincialis (Bivalvia, Mollusca) exposed to hypoxia
Author(s)
Woo, Seonock; Denis, Vianney; Won, Hyokyung; Shin, Kyungsoon; Lee, Gunsup; Lee, Taek-Kyun; Yum, Seungshic
KIOST Author(s)
Shin, Kyoung Soon(신경순)Lee, Taek Kyun(이택견)Yum, Seung Shic(염승식)
Alternative Author(s)
우선옥; 원효경; 신경순; 이건섭; 이택견; 염승식
Publication Year
2013-09
Abstract
Background: In this study, we investigated transcription and enzyme level responses of mussels Mytilus galloprovincialis exposed to hypoxic conditions. Genes for catalase (CAT), cytochrome P450, glutathione S-transferase (GST), metallothionein, superoxide dismutase (SOD), cytochrome c oxidase subunit 1 (COX-1), and NADH dehydrogenase subunit 2 were selected for study. Transcriptional changes were investigated in mussels exposed to hypoxia for 24 and 48 h and were compared to changes in control mussels maintained at normal oxygen levels. Activities of CAT, GST, and SOD enzymes, and lipid peroxidation (LPO) were also investigated in mussels following exposure to hypoxia for 24, 48, and 72 h. Results: Relative to the control group, the CAT activity decreased in all hypoxia treatments, while the activity of GST significantly increased in mussels exposed to hypoxia for 24 and 48 h, but decreased in those exposed for 72 h. The LPO levels were significantly higher in mussels in the 24- and 48-h hypoxia treatments than those in the control mussels, but there was no significant change in the SOD activities among all hypoxia treatments. Messenger RNA levels for the CAT, cytochrome P450, GST, metallothionein, and SOD genes were not significantly affected by hypoxic conditions for 48 h, but the expressions of the COX-1 and NADH dehydrogenase subunit 2 genes were significantly repressed in mussels in both the 24- and 48-h exposure treatments. Conclusions: These results demonstrate the transcriptional stability and changes among several genes related to oxidative stress under oxygen-depletion conditions in M. galloprovincialis and provide useful information about the modulation of antioxidant enzyme activities induced by hypoxia in a marine animal.
ISSN
1021-5506
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3116
DOI
10.1186/1810-522X-52-15
Bibliographic Citation
ZOOLOGICAL STUDIES, v.52, 2013
Publisher
BIODIVERSITY RESEARCH CENTER, ACAD SINICA
Subject
GLUTATHIONE S-TRANSFERASES; RESPONSES; MUSSEL; CONSUMPTION; INDUCTION
Keywords
Mytilus galloprovincialis; Hypoxia; Oxidative stress-related gene; Antioxidant enzymes
Type
Article
Language
English
Document Type
Article
Publisher
BIODIVERSITY RESEARCH CENTER, ACAD SINICA
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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