새로운 수계 위해성 평가 시험어종-자바송사리

Title
새로운 수계 위해성 평가 시험어종-자바송사리
Alternative Title
Javanese Medaka, Oryzias javanicus, a New Test Organism for Aquatic Risk Assessment
Author(s)
우선옥; 염승식; 이택견
KIOST Author(s)
Yum, Seungshic(염승식)Lee, Taek Kyun(이택견)
Publication Year
2006-05-15
Abstract
Physiological studies in recenet years have shown the high adaptability from freshwater to seawater of of Javanese Medaka, Oryzias javanicus. Results indicate O. javanicus is a unique and good candidate as a test fish in both marine and freshwater environments and may deliver new insights into our knowledge of aquatic risk assessment. To develop molecular biomarkers for toxicity assessment, we have obtained six stress-responsive genes [metallothionein (MT), catalase (CAT), superoxide dismutase (SOD), glutathion S-transferase (GST), cytochrome P-450 (CYP1A1), uniquitin (UBI)] from O. javanicus and investigated their expression changes in fishes exposed to a variety of concentrations of cadmium (Cd). More detailed observations on differential expression of MT gene after heavy metal exposure were carried out as follows. The pattern of OjaMT expression induced by six heavy metals was analyzed by real-time quantitative PCR. The level of hepatic OjaMT mRNA was increased in a dose-dependent manner by Ag, Cd, Cu, and Zn after 24 h of exposure. However, after Cr and Ni exposure, a significant decrease in OjaMT levels was observed. Cadmium-induced OjaMT expression was detectable in fishes as young as 3 months. After Cd exposure, OjaMT induction was prominent in intestine and liver and intermediate in muscle and gill. OjaMT mRNA levels could representa good biomarker for monitoring heavy metals in seawater.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/31093
Bibliographic Citation
한국해양학회, pp.210 - 215, 2006
Publisher
한국해양학회
Type
Conference
Language
English
Publisher
한국해양학회
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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