연산호군락의 건강평가를 위한 기능유전체적 접근

Abstract

The extensive gene isolation of potentially responsive to stressful conditions from a soft coral Scleronephthya gracillimum is described. Soft coral colonies were exposed to thermal and chemical stressors to induce the expression of stress related genes. Differentially expressed genes by natural or anthropogenic stressors were identified by construction of standard and stress exposed-paired subtractive cDNA library; and differential display (DD-) PCR. Thirty-four and thirty-seven kinds of candidate genes were identified from thermal or benzo[a]pyrene stress exposed group, respectively, which associated with cell cycle, cell signalling, transcription, translation, protein metabolism, and other cellular functions. In the case of DD-PCR analysis, 334 differentillay expressed genes which induced by natural (thermal) or xenobiotic (PAHs) stressor were isolated. The isolated and identified differentially expressed genes have great potential for identifying environmental stressors and may open a new paradigm on soft coral health assessment.