서식처 특성에 따른 신규 미생물의 다양성

Title
서식처 특성에 따른 신규 미생물의 다양성
Alternative Title
HABITAT SPECIFICITY OF MARINE MICROBIAL NOVEL DIVERSITY
Author(s)
권개경; 양성현; 임재규; 배승섭; 김준태; 강지현; 이희순; 정홍배; 김윤재; 황영옥; 강성균; 이정현; 김상진
KIOST Author(s)
Kwon, Kae Kyoung(권개경)Kang, Sung Gyun(강성균)Lee, Jung Hyun(이정현)
Publication Year
2006-08-22
Abstract
Diverse microorganisms are found in marine environment, displaying various physico-chemical ecosystems which are three dimensional, however, many of which still not identified. Since 1990s, we has been cruise a lot of regions of various marine environments such as coastal areas, deep sea including thermal vent and cold-seep, tropical sea and cold regions like Antarctic and Arctic, and more than 3,000 microorganisms were isolated and preserved. The molecular phylogenetic analysis of the isolates led to some interesting affirmative conclusions. To isolate marine bacteria, collected samples such as sediment, seawater or organisms were spread onto ZoBell 2216e agar plate without or with enrichment in various organic substrates such as starch, skim milk, tributyrin, etc. Colonies showing different morphotypes were selected and analyzed by 16S rRNA gene sequencing to determine taxonomic position. As a result, it was found that app. 10% was not similar to the known bacteria, showing the similarity lower than 96% in 16S rRNA sequence, and assumed as novel genus or species. Majority of isolates were classified into gamma-Proteobacteria (42%), followed by Gram-positive bacteria (37%), alpha-Proteobacteria (14%) and CFB-group (7%). Conclusively, most of novel strains from cold seep were affiliated into gamma-Proteobacteria, and novel isolates from open sea into Gram-positive bacteria and alpha-Proteobacteria while novel CFB-group bacteria were mainly isolated from polar region. Novel isolates from coastal environments evenly belong to the four taxonomic groups with a slightly higher probability of grouping into alpha-Proteobacteria. These results imply that a marine environment provides a special ecosystem preferred by a specific group.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/30919
Bibliographic Citation
11th International Symposium on Microbial Ecology, pp.202, 2006
Publisher
International Society of Microbial Ecology
Type
Conference
Language
English
Publisher
International Society of Microbial Ecology
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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