열대해역 마이크로네시아 축주와 온대해역 한국 태종대에 서식하는 해면공생 배양미생물의 군집조성 비교

Abstract

Sponge-associated microorganisms has been highlighted due to their possibility of producing novel bioactive products. Many sponge-associated bacterial strains isolated from tropic area and temperate area for further utilization. Based on the 16S rRNA gene sequences, compositional difference between isolates from tropical area, Chuuk Island, Micronesia, and from temperate area, Taejongdae, Korea, was compared. Majority of isolates from temperate area belonging to the alpha-proteobacteria distributed various genera include the genus Pseudovibrio in the family Rhodobacteraceae and in the family Erythrobacteraceae. However, majority of isolates from tropical area affiliated only into the genus Pseudovibrio, even more, produce different clade with that of temperate isolates in the same genus. Isolates belonging to the gamma-proteobacteria from temperate area affiliated into the genera Bowmanella, Pseudoalteromonas, Shewanella and Vibrio, however, from tropical area affiliated into the genera Acinetobacter, Halomonas, Microbulbifer and Pseudomonas. In case of Bacteroidetes, isolates discriminated clearly as Flavobacteria (Temperate isolates) or Sphingobacteria (tropical isolates). The generic difference of sponge-associated was shown between Taejongdae isolates and Chuuk Island isolates, however, is it due to the different temperature or due to the site difference was unclear.