Differential gene expression induced by heavy metal in marine medaka, Oryzias javanicus
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Title
- Differential gene expression induced by heavy metal in marine medaka, Oryzias javanicus
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Author(s)
- 우선옥; 염승식; 이택견
- KIOST Author(s)
- Woo, Seon Ock(우선옥); Yum, Seung Shic(염승식); Lee, Taek Kyun(이택견)
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Alternative Author(s)
- 우선옥; 염승식; 이택견
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Publication Year
- 2006-11-06
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Abstract
- A metallothionein (MT) gene was isolated for the first time from Javanese medaka, Oryzias javanicus, which shows high adaptability from freshwater to seawater. The full-length cDNA of MT from O. javanicus (OjaMT) comprises 349 bp, excluding the poly(A)+ stretch, and codes for a total of 60 amino acids. The positions of cysteine residues are highly conserved. The pattern of OjaMT expression induced by six heavy metals was analyzed by real-time quantitative PCR. The level of hepatic OjaMT mRNA was increased in a dose-dependent manner by Ag, Cd, Cu, and Zn after 24 h of exposure. However, after Cr and Ni exposure, a significant decrease in OjaMT levels was observed. Cadmium-induced OjaMT expression was detectable in fishes as young as 3 months. After Cd exposure, OjaMT induction was prominent in intestine and liver and moderate in muscle and gill. OjaMT mRNA levels could represent a good biomarker for monitoring heavy metals in seawater.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/30771
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Bibliographic Citation
- 2006 대한독성유전단백체학회 국제학술대회, pp.398, 2006
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Publisher
- 대한독성유전단백체학회
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Type
- Conference
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Language
- English
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