Differential gene expression induced by heavy metal in marine medaka, Oryzias javanicus

Title
Differential gene expression induced by heavy metal in marine medaka, Oryzias javanicus
Author(s)
우선옥; 염승식; 이택견
KIOST Author(s)
Yum, Seung Shic(염승식)Lee, Taek Kyun(이택견)
Publication Year
2006-11-06
Abstract
A metallothionein (MT) gene was isolated for the first time from Javanese medaka, Oryzias javanicus, which shows high adaptability from freshwater to seawater. The full-length cDNA of MT from O. javanicus (OjaMT) comprises 349 bp, excluding the poly(A)+ stretch, and codes for a total of 60 amino acids. The positions of cysteine residues are highly conserved. The pattern of OjaMT expression induced by six heavy metals was analyzed by real-time quantitative PCR. The level of hepatic OjaMT mRNA was increased in a dose-dependent manner by Ag, Cd, Cu, and Zn after 24 h of exposure. However, after Cr and Ni exposure, a significant decrease in OjaMT levels was observed. Cadmium-induced OjaMT expression was detectable in fishes as young as 3 months. After Cd exposure, OjaMT induction was prominent in intestine and liver and moderate in muscle and gill. OjaMT mRNA levels could represent a good biomarker for monitoring heavy metals in seawater.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/30771
Bibliographic Citation
2006 대한독성유전단백체학회 국제학술대회, pp.398, 2006
Publisher
대한독성유전단백체학회
Type
Conference
Language
English
Publisher
대한독성유전단백체학회
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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