Induction of Apoptosis by the Tropical Seaweed Pylaiella littoralis in HT-29 Cells via the Mitochondrial and MAPK Pathways SCIE SCOPUS KCI

DC Field Value Language Ye, Bo-Ram - Kim, Junseong - Kim, Min-Sun - Jang, Jiyi - Oh, Chulhong - Kang, Do-Hyung - Qian, Zhong-Ji - Jung, Won-Kyo - Choi, Il-Whan - Heo, Soo-Jin - 2020-04-20T05:25:15Z - 2020-04-20T05:25:15Z - 2020-01-28 - 2013-12 -
dc.identifier.issn 1738-5261 -
dc.identifier.uri -
dc.description.abstract We demonstrated that an extract from Pylaiella littoralis, collected from the Federate States of Micronesia (FSM), could inhibit the proliferation of tumor cells. P. littoralis extract (PLE) showed anti-proliferative activities in the tumorigenic cells tested, ranging from 20.2% to 67.9%. The highest inhibitory activity, in HT-29 cells, was selected for further experiments. PLE showed no cytotoxic effect in normal cells and inhibited the growth of HT-29 cells depending on concentration and incubation time. PLE-treated HT-29 cells showed the typical morphological characteristics of apoptosis, such as apoptotic body formation and DNA fragmentation. PLE also induced mitochondrial membrane potential depolarization and resulted in increased mitochondrial membrane permeability, compared with untreated cells. PLE decreased Bcl-2 protein and increased Bax protein expression, activating caspase-3 and poly (ADP-ribose) polymerase (PARP) expression via the caspase pathway. PLE also increased the phosphorylation of c-Jun N-terminal kinase (JNK), p38, and extracellular signal-regulated kinase (ERK), and it reduced cell viability in treatment cells with specific inhibitors such as PD98059 (a specific inhibitor of ERK), SP600125 (a specific inbibitor of JNK), and SB 203580 (a specific inbibitor of p38 MAPK). via the the mitogen-activated protein kinases (MAPKs) pathway. These results suggest that PLE inhibits the proliferation of HT-29 cells by affecting the caspase and MAPK pathways involved in the induction of apoptosis. Thus, we suggest that P. littoralis extract might be potential candidate agents for the treatment of human colorectal cancer. -
dc.description.uri 1 -
dc.language English -
dc.subject LEUKEMIA HL-60 CELLS -
dc.subject CYTOCHROME-C -
dc.subject HELA-CELLS -
dc.subject BROWN ALGA -
dc.subject IN-VITRO -
dc.subject ACTIVATION -
dc.subject DEATH -
dc.subject BCL-2 -
dc.subject ZOOPLANKTON -
dc.title Induction of Apoptosis by the Tropical Seaweed Pylaiella littoralis in HT-29 Cells via the Mitochondrial and MAPK Pathways -
dc.type Article -
dc.citation.endPage 348 -
dc.citation.startPage 339 -
dc.citation.title OCEAN SCIENCE JOURNAL -
dc.citation.volume 48 -
dc.citation.number 4 -
dc.identifier.bibliographicCitation OCEAN SCIENCE JOURNAL, v.48, no.4, pp.339 - 348 -
dc.identifier.doi 10.1007/s12601-013-0032-z -
dc.identifier.scopusid 2-s2.0-84891308345 -
dc.identifier.wosid 000335400700005 -
dc.type.docType Article -
dc.identifier.kciid ART001827231 -
dc.description.journalClass 1 -
dc.subject.keywordPlus LEUKEMIA HL-60 CELLS -
dc.subject.keywordPlus CYTOCHROME-C -
dc.subject.keywordPlus ANTIOXIDANT PROPERTIES -
dc.subject.keywordPlus HELA-CELLS -
dc.subject.keywordPlus BROWN ALGA -
dc.subject.keywordPlus IN-VITRO -
dc.subject.keywordPlus ACTIVATION -
dc.subject.keywordPlus DEATH -
dc.subject.keywordPlus BCL-2 -
dc.subject.keywordPlus ZOOPLANKTON -
dc.subject.keywordAuthor Federated States of Micronesia -
dc.subject.keywordAuthor Pylaiella littoralis -
dc.subject.keywordAuthor HT-29 cell -
dc.subject.keywordAuthor apoptosis -
dc.subject.keywordAuthor anti-proliferative activity -
dc.relation.journalWebOfScienceCategory Marine & Freshwater Biology -
dc.relation.journalWebOfScienceCategory Oceanography -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.description.journalRegisteredClass kci -
dc.relation.journalResearchArea Marine & Freshwater Biology -
dc.relation.journalResearchArea Oceanography -
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Jeju Research Institute > Jeju Marine Research Center > 1. Journal Articles
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