Contrasting effects of 4-nonlyphenol on the gene expression of vitellogenin and retinol-binding protein in a rockfish Sebastes schlegeli

Title
Contrasting effects of 4-nonlyphenol on the gene expression of vitellogenin and retinol-binding protein in a rockfish Sebastes schlegeli
Author(s)
배선혜; 정지현; 이재용; 김명희; 한창희
KIOST Author(s)
Jung, Jee Hyun(정지현)
Publication Year
2007-06-03
Abstract
Retinol-binding protein (RBP) plays an important role in the specific plasma transport of retinol to target cells in higher vertebrates. In order to clarify the effects of 4-nonylphenol (4-NP) on RBP mRNA expression in the rockfish, Sebastes schlegeli which is common in coastal waters of Korea and commercially important species, the cDNA library was constructed from liver, and the partial RBP were encoded. The deduced amino acid sequence of 146 bases RBP mRNA showed a high homology to Sparus aurata (80%), Oncorhynchus mykiss (72%), Anguilla anguilla (78%). Effects of 4-NP on RBP and VTG mRNA expression level in rockfish were examined by the northern blot analysis. In female and male rockfish injected with 4-NP of 10 mg/kg BW, there was no changes in the levels of VTG mRNA expression. On the other hand the RBP mRNA levels were reduced at 48 hours after injection in male. In rockfish injected with 4-NP 25 mg/kg BW, the level of VTG mRNA expression increased after 24 hours, regardless of sex. The level of RBP mRNA expression was reduced 48 hours after administration. These data indicate that estrogenic mimics such as 4-NP exhibits contrasting effect on RBP and VTG gene expression in rockfis
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/30458
Bibliographic Citation
5 th ICOMPE, pp.115, 2007
Publisher
City of Hongkong university
Type
Conference
Language
English
Publisher
City of Hongkong university
Related Researcher
Research Interests

Toxicology,Fish physiology,Marine environment risk assessment,독성학,어류생리학,해양환경 위해성평가

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