유기인계 살생제에 의한 자바송사리의 유전자 발현변화

Abstract

In this study we used the stress responsive genes such as catalase (CAT), cytochrome P450 (CYP1A), glucose-6-phosphate dehydrogenase (G6PD), glutathione peroxidase (GPX), glutathione reductase (GR), glutathione S-transferase (GST), superoxide dismutase (SOD) and ubiquitin (UB) as biomarkers to investigate the effects of organic pollutant on transcriptional changes in intestine, liver and muscle of Javanese medaka fish (Oryzias javanicus). Fishes were exposed to a variety of concentrations of organophosphorus pesticide, iprobenfos, then the organic pollutant induced transcriptional changes of 8 stress responsive genes in 3 tissues were analyzed by using quantitative RT-PCR. Pretreatment of animals in vivo with iprobenfos produced a marked induction of CAT, GPX, GR and UB gene expression in liver and also CYP1A, GST, G6PD and SOD gene expressions in liver increased significantly comparing with non-exposed control fish. In particular, gene expressions in muscle did not show a significant change in low concentration of exposure. These results suggested that transcriptional changes of those 8 genes could be good biomarkers to detect stress levels in Oryzias javanicus.