Neptunomonas acidivorans sp. nov., Isolated from sediment, And emended description of the genus Neptunomonas

Abstract

A Gram-reaction-negative, aerobic, rod-shaped (1.2-1.6 mm_0.6-0.8 mm), flagellated and motile marine bacterium, designated MEBiC06243T, was isolated from a sediment collected at Daebu Island in the Yellow Sea (376 209 N 1266 419 E), Korea. The 16S rRNA gene sequence analysis revealed that strain MEBiC06243Tshowed high similarity with Neptunomonas naphthovorans NAG-2N-126T(96.3 %). Growth was observed at 10-39 6C (optimum 29 6C), at pH 6.0-9.0 (optimum pH 7) and with 0-7% (optimum 2.5 %) NaCl. The predominant cellular fatty acids were C10 : 03-OH (6.1 %), C12 : 0(5.8 %), C16 : 0(30.5 %), C18 : 1v7c (21.6 %) and summed feature 3 (comprising C15 : 02-OH and/or C16 : 1ω7c; 30.7 %). The DNA G+C content was 41.4 mol%. The major respiratory quinone was Q-8. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified lipids, one unidentified aminophospholipid and three unidentified aminolipids were detected as major polar lipids. On the basis of this polyphasic taxonomic data, strain MEBiC06243T should be classified as a novel species of the genus Neptunomonas proposed as Neptunomonas acidivorans sp. nov. The type strain is MEBiC06243T(=KCCM 42975T=JCM 18291T). An emended description of the genus Neptunomonas is also given. © 2014 IUMS.