서로다른 추출방법으로 부터 추출된 녹조류의 잠재적 생리활성 평가

Abstract

This study was conducted to screen potential bioactivities including antioxidant, anti-hypertension, and anticancer activities of methanol and aqueous extracts from 10 marine green algae collected along the coast of Jeju Island in Korea. The extracts were prepared at 20 and 70°C temperature conditions and applied in different bioassays. A variety of extracts evidenced positive effect against reactive oxygen species (ROS), angiotensin converting enzyme (ACE), and various cancer cell lines. Among the algae, Ulva pertusa evidenced prominent inhibitory activity against harmful factors compared with other algae tested in this study, and it is available in large quantities as well as easy to collect. Therefore, U. pertusa was selected for further study. U. pertusa was converted into dried form using different drying methods such as far infrared radiation drying, freeze drying, and okadora drying which were extracted using ethanol and water. Most of the tested extracts evidenced positive effect against ROS whereas the aqueous extract from U. pertusa dried by okadora exhibited higher activity in both 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide (MTT) assays. In addition, U. pertusa was digested by food grade enzymes, and was examined their antioxidant and anticancer activities. The enzymatic digests too evidenced positive effect on both, ROS and anticancer assays. Therefore, these results indicated that green algae could be used as natural source which have potential bioactivities, and further investigations are needed to identify their active components responsible for the potential activities.