6,7-DIMETHOXY-4-METHYLCOUMARIN SUPPRESSES PRO-INFLAMMATORY MEDIATOR EXPRESSION THROUGH INACTIVATION OF THE NF-kappa B AND MAPK PATHWAYS IN LPS-INDUCED RAW 264.7 CELLS SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Kim, K.-N. | - |
dc.contributor.author | Yang, H.-W. | - |
dc.contributor.author | Ko, S.-C. | - |
dc.contributor.author | Ko, Y.-J. | - |
dc.contributor.author | Kim, E.-A. | - |
dc.contributor.author | Roh, S.W. | - |
dc.contributor.author | Ko, E.-Y. | - |
dc.contributor.author | Ahn, G. | - |
dc.contributor.author | Heo, S.-J. | - |
dc.contributor.author | Jeon, Y.-J. | - |
dc.contributor.author | Yoon, W.-J. | - |
dc.contributor.author | Hyun, C.-G. | - |
dc.contributor.author | Kim, D. | - |
dc.date.accessioned | 2020-04-20T04:55:22Z | - |
dc.date.available | 2020-04-20T04:55:22Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1611-2156 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/2926 | - |
dc.description.abstract | In this study, we investigated the ability of 6,7-dimethoxy-4-methylcoumarin (DMC) to inhibit lipopolysaccharide (LPS)-induced expression of pro-inflammatory mediators in mouse macrophage (RAW 264.7) cells, and the molecular mechanism through which this inhibition occurred. Our results indicated that DMC downregulated LPS-induced nitric oxide (NO) synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, thereby reducing the production of NO and prostaglandin E2 (PGE2) in LPS-activated RAW 264.7 cells. Furthermore, DMC suppressed LPS-induced production ofpro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α. To elucidate the mechanism underlying the antiinflammatory activity of DMC, we assessed itseffects on the mitogen-activated protein kinase (MAPK) pathway and the activity and expression of nuclear transcription factor kappa-B (NF-κB). The experiments demonstrated that DMC inhibited LPS-induced phosphorylation of extracellular signal-regulated kinases (ERKs), c-Jun N-terminal kinase (JNK), and p38. In addition, it attenuated LPS-induced NF-κB activation via the inhibition of IκB-α phosphorylation. Taken together, these data suggest that DMC exerts its anti-inflammatory effects in RAW 264.7 cells through the inhibition of LPS-stimulated NF-κB and MAPK signaling, thereby downregulating the expression of pro-inflammatory mediators. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | Leibniz Research Centre for Working Environment and Human Factors | - |
dc.title | 6,7-DIMETHOXY-4-METHYLCOUMARIN SUPPRESSES PRO-INFLAMMATORY MEDIATOR EXPRESSION THROUGH INACTIVATION OF THE NF-kappa B AND MAPK PATHWAYS IN LPS-INDUCED RAW 264.7 CELLS | - |
dc.type | Article | - |
dc.citation.endPage | 800 | - |
dc.citation.startPage | 792 | - |
dc.citation.title | EXCLI Journal | - |
dc.citation.volume | 13 | - |
dc.contributor.alternativeName | 허수진 | - |
dc.identifier.bibliographicCitation | EXCLI Journal, v.13, pp.792 - 800 | - |
dc.identifier.scopusid | 2-s2.0-84904998095 | - |
dc.identifier.wosid | 000341518200025 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordPlus | stress activated protein kinase 1 | - |
dc.subject.keywordPlus | synaptophysin | - |
dc.subject.keywordPlus | tumor necrosis factor alpha | - |
dc.subject.keywordPlus | unclassified drug | - |
dc.subject.keywordPlus | animal cell | - |
dc.subject.keywordPlus | animal cell culture | - |
dc.subject.keywordPlus | antiinflammatory activity | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | cell line | - |
dc.subject.keywordPlus | cell viability | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | cytokine production | - |
dc.subject.keywordPlus | down regulation | - |
dc.subject.keywordPlus | drug structure | - |
dc.subject.keywordPlus | enzyme phosphorylation | - |
dc.subject.keywordPlus | macrophage | - |
dc.subject.keywordPlus | mouse | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | protein analysis | - |
dc.subject.keywordPlus | protein expression | - |
dc.subject.keywordPlus | protein synthesis inhibition | - |
dc.subject.keywordPlus | RAW 264.7 cell | - |
dc.subject.keywordPlus | signal transduction | - |
dc.subject.keywordPlus | 6,7 dimethoxy 4 methylcoumarin | - |
dc.subject.keywordPlus | antiinflammatory agent | - |
dc.subject.keywordPlus | cyclooxygenase 2 | - |
dc.subject.keywordPlus | immunoglobulin enhancer binding protein | - |
dc.subject.keywordPlus | interleukin 1beta | - |
dc.subject.keywordPlus | interleukin 6 | - |
dc.subject.keywordPlus | lipopolysaccharide | - |
dc.subject.keywordPlus | mitogen activated protein kinase | - |
dc.subject.keywordPlus | nitric oxide synthase | - |
dc.subject.keywordPlus | prostaglandin E2 | - |
dc.subject.keywordAuthor | 6,7-Dimethoxy-4-methylcoumarin (DMC) | - |
dc.subject.keywordAuthor | Anti-inflammatory | - |
dc.subject.keywordAuthor | MAPKs | - |
dc.subject.keywordAuthor | NF-κB | - |
dc.subject.keywordAuthor | RAW 264.7 cells | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |