Molecular cloning and expression analysis of two lipopolysaccharide-induced TNF-alpha factors (LITAFs) from rock bream, Oplegnathus fasciatus SCIE SCOPUS

Cited 13 time in WEB OF SCIENCE Cited 13 time in Scopus
Title
Molecular cloning and expression analysis of two lipopolysaccharide-induced TNF-alpha factors (LITAFs) from rock bream, Oplegnathus fasciatus
Author(s)
Hwang, Seong Don; Shim, Sang Hee; Kwon, Mun-Gyeong; Chae, Young Sun; Shim, Won Joon; Jung, Jee-Hyun; Kim, Ju-Won; Park, Chan-Il
KIOST Author(s)
Shim, Won Joon(심원준)Jung, Jee Hyun(정지현)
Publication Year
2014-02
Abstract
Lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-alpha factor (LITAF) plays an important role controlling the expression of TNF-alpha and the other cytokine genes in the presence of LPS. However, two LITAF homologues have not been characterized in fish. In this study, we cloned two distinct LITAF (RbLITAF1 and RbLITAF2) cDNAs from rock bream (Oplegnathus fasciatus) and characterized their expression profiles after infection with Edwardsiella tarda, Streptococcus iniae or red seabream iridovirus (RSIV). The coding regions of RbLITAF1 and RbLITAF2 cDNAs were 492 bp and 417 bp, encoding 153 and 138 amino acid residues, respectively. The genes consisted of a LITAF domain. RbLITAF1 was highly expressed in the spleen and heart of healthy rock bream, whereas RbLITAF2 was highly expressed in the gill, intestine and stomach. In spleen, the gene expression of RbLITAF1 and RbLITAF2 were increased until 5 days post-infection (dpi), and then decreased at 7 dpi. In kidney, E. tarda and RSIV infection led to induction of the RbLITAF1 gene at 1 dpi, RbLITAF2 gene was down-regulated after pathogen infection. These results suggest that RbLITAFs may be involved in the LITAF-mediated immune response and regulate systemic immune responses against pathogen infection. (C) 2014 Elsevier Ltd. All rights reserved.
ISSN
1050-4648
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2909
DOI
10.1016/j.fsi.2013.12.023
Bibliographic Citation
FISH & SHELLFISH IMMUNOLOGY, v.36, no.2, pp.467 - 474, 2014
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Subject
TUMOR-NECROSIS-FACTOR; NF-KAPPA-B; GRASS CARP REOVIRUS; TRANSCRIPTION FACTOR; INNATE IMMUNITY; SIGNALING PATHWAY; GENE-EXPRESSION; LPS; CACHECTIN; PROTEIN
Keywords
LITAF; Oplegnathus fasciatus; Edwardsiella tarda; Streptococcus iniae; Red seabream iridovirus
Type
Article
Language
English
Document Type
Article
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Related Researcher
Research Interests

Microplastic pollution,Persistent Organic Pollutants,Oil Pollution,미세플라스틱 오염,잔류성 유기오염물질,유류오염

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