Tenacibaculum luteolum sp. nov., Isolated from a Marine Sponge

Abstract

A novel psychrotrophic bacterium, strain HJ103T, was isolated from a marine sponge collected in the East Sea, Korea (also known as Sea of Japan). Cells were Gram-negative, motile by gilding, strictly aerobic, catalase-, and oxidase-positive, rod-shaped (0.3-0.5㎛ ´ 1.6-6.2㎛) and halophilic. Growth was observed between 5 and 33℃ (optimum 26℃), at pH 6.5-9.0 (optimum 8.0) and in the presence of 2.0-3.5% (optimum 2.5%) NaCl. The strain requires Ca2+, Mg2+ and K+ ions for growth. The 16S rRNA gene sequence analysis revealed that strain HJ103T showed high similarity with members of the genus Tenacibaculum (94.4-96.4%), and phylogenetic analysis revealed that strain HJ103T shared a phyletic line with Tenacibaculum maritimum. The major respiratory quinone is MK-6. The DNA G+C content was 31.1 mol%. The major fatty acids were iso-C15:0, iso-C15:0 3-OH, C16:0 3-OH and summed feature 3 comprising C16:1 w7c and/or iso-C15:0 2-OH. On the basis of this polyphasic taxonomic evidence, strain HJ103T should be classified as a species in the genus Tenacibaculum and the name as Tenacibaculum luteolum sp. nov. is proposed. The type strain is HJ103T (= KCCM 42328T = JCM 14618T).