Screening of beta-glucosidase genes from marine bacteria

Abstract

Beta-glucosidase is a glucosidase enzyme that acts upon β1→4 bonds linking two glucose or glucose-substituted molecules. The enzyme occurs ubiquitously in plants, animals, fungi and bacteria which convert cellubiose to glucose. Cellulase (beta-1,4-glucanase and beta-glucosidase) catalyzing the hydrolysis of plant polysaccharides are industrially important enzymes used to saccharify industrial and agricultural cellulose-containg residues, treat cellulose pulp wastes in the paper industry, enhance the extraction of fermentable substances in the brewing and alcohol fermentation inducstries. Beta-glucosidase producing bacteria were screened which the samples were collected in Chuuk (Micronesia) and Jeju (Republic of Korea). The strains were identified with 16s rRNA sequences. We were designed several primers based on each identified strains for amplification of patial beta-glucosidase sequences. PCR were carried out with each differant conditions. The purified PCR products were ligated into pGEM easy T vector and transformed into E. coli DH5alpha. Plasmid DNA were extracted with Plasmid DNA extraction Kit and the sequencing was carried out by Solgent inc. (Korea). The beta-glucosidase nucleotide sequences were analyzed by nucleotide Blast (NCBI) and DNassist 2.2 programs. We found 13 new beta-glucosidase sequences. The stains of analyzed genes were included as Glaciecola mesophila, Alteromonas macleodii, Alteromonas alvinellae, Shewanella algae, Vibrio harveyi, Vibrio campbellii, Vibrio natriegens, Vibrio parahaemolyticus, Pseudoalteromonas elyakovii, Paracoccus zeaxanthinifaciens, Aestuariibacter sp., Oceanicola sp..