Fucoxanthin induces apoptosis in human leukemia HL-60 cells through a ROS-mediated MAPK pathway
DC Field | Value | Language |
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dc.contributor.author | 허수진 | - |
dc.contributor.author | 오철홍 | - |
dc.contributor.author | Abu Affan | - |
dc.contributor.author | 김길남 | - |
dc.contributor.author | 강도형 | - |
dc.date.accessioned | 2020-07-16T19:30:31Z | - |
dc.date.available | 2020-07-16T19:30:31Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2010-10-10 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/28739 | - |
dc.description.abstract | Fucoxanthin, a natural biologically active substance isolated from Ishige okamurae, has potent antitumor activity of human leukemia cell HL-60 cells via apoptosis induction. However, the mechanism underlying fucoxanthin-induced apoptosis in HL-60 cells remains unclear. In the present study, we focused on the effect of fucoxanthin induction on reactive oxygen species (ROS) accumulation, and triggering the mitogen-activated protein kinase (MAPKs) signaling pathway in HL-60 cells. We found that ROS are generated during fucoxanthin-induced cytotoxicity and apoptosis in HL-60 cells, and N-acetylcysteine (NAC) which is a scavenger of ROS, suppressed fucoxanthin-induced cytotoxicity and apoptosis. Moreover, fucoxanthin dramatically increased phosphorylation of c-Jun N-terminal kinase (JNK) and p38 kinase when compared to the treatment without fucoxanthin but the treatment with NAC dramatically inhibited fucoxanthin-induced phosphorylation of JNK and p38 kinase in HL-60 cells. In addition, fucoxanthin-induced the cleavage of caspase-3, -7, and poly-ADP-ribose polymerase (PARP) and decrease of Bcl-xL levels, whereas NAC pre-treatment significantly inhibited the cleavage of caspase-3, -7, and PARP and decrease of Bcl-xL levels. In this study it was firstly demonstrated that fucoxanthin generated ROS and the accumulation of ROS played an important role in fucoxanthin-induced JNK/p38 MAPK signaling pathway, which provided further theoretical support for the application of fucoxanthin as a promising anticancer agent. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | International Marine Biotechnology Association | - |
dc.relation.isPartOf | Ninth International Marine Biotechnology Conference | - |
dc.title | Fucoxanthin induces apoptosis in human leukemia HL-60 cells through a ROS-mediated MAPK pathway | - |
dc.type | Conference | - |
dc.citation.conferencePlace | CC | - |
dc.citation.endPage | 442 | - |
dc.citation.startPage | 442 | - |
dc.citation.title | Ninth International Marine Biotechnology Conference | - |
dc.contributor.alternativeName | 허수진 | - |
dc.contributor.alternativeName | 오철홍 | - |
dc.contributor.alternativeName | Abu Affan | - |
dc.contributor.alternativeName | 강도형 | - |
dc.identifier.bibliographicCitation | Ninth International Marine Biotechnology Conference, pp.442 | - |
dc.description.journalClass | 1 | - |