Neuroprotective Effect of Diphlorethohydroxycarmalol Isolated from Ishige okamurae against H2O2-Induced Oxidative Stress in Neuronal HT22 Cells

Abstract

The present study is designed to investigate the neuroprotective effect of a kind of phlorotannins, diphlorethohydroxycarmalol (DPHC) isolated from Ishige okrmurae against hydrogen peroxide (H2O2)-induced oxidative stress in HT22 murine hippocampal neuronal cells. H2O2 treatment induced neurotoxicity whereas DPHC prevented cells from H2O2-induced damage then restoring cell viability was significantly increased. Intracellular reactive oxygen species (ROS) and lipid peroxidation was overproduced as the result of the addition of H2O2, however, these ROS generation and lipid peroxidation was effectively inhibited by addition of DPHC in a dose-dependent manner. Moreover, DPHC suppressed the elevation of H2O2-induced Ca2+ release. These findings indicate that DPHC has neuroprotective effects against H2O2-induced damage in neuronal cells, and that an inhibitory effect on ROS production may contribute to the underlying mechanisms.