Structural Basis for the Speciallization of Nur in Metal Sensing and DNA Recognition

Title
Structural Basis for the Speciallization of Nur in Metal Sensing and DNA Recognition
Author(s)
차선신
Publication Year
2011-06-13
Abstract
Nur, a member of the Fur family, is a nickel-responsive transcription factor that controls nickel homeostasis and anti-oxidative response in Streptomyces coelicolor. Here we report the 2.4 Å resolution crystal structure of Nur. It contains a unique nickel-specific metal site in addition to a non-specific common metal site. The identification of the 6-5-6 motif of the Nur recognition box and a Nur/DNA complex model reveals that Nur mainly interacts with terminal bases of the palindrome on complex formation. This contrasts with more distributed contacts between Fur and the n-1-n type of the Fur binding motif. The disparity between Nur and Fur in the conformation of the S1-S2 sheet in the DNA-binding domain can explain their different DNA-recognition patterns. Furthermore, the fact that the specificity of Nur in metal sensing and DNA recognition is conferred by the specific metal site suggests that its introduction drives the evolution of Nur orthologs in the Fur family.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/28301
Bibliographic Citation
2011 international meeting of the microbiological society of korea, 2011
Publisher
대한미생물학회
Type
Conference
Language
English
Publisher
대한미생물학회
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