Chromene induces apoptosis via caspase-3 activation in human leukemia HL-60 cells

DC Field Value Language
dc.contributor.author 예보람 -
dc.contributor.author 김태호 -
dc.contributor.author 정주영 -
dc.contributor.author 전선미 -
dc.contributor.author 권영경 -
dc.contributor.author 장지이 -
dc.contributor.author Abu Affan -
dc.contributor.author 오철홍 -
dc.contributor.author 강도형 -
dc.contributor.author Jung-Ho Hyun -
dc.contributor.author 허수진 -
dc.date.accessioned 2020-07-16T15:31:12Z -
dc.date.available 2020-07-16T15:31:12Z -
dc.date.created 2020-02-11 -
dc.date.issued 2011-08-16 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/28244 -
dc.description.abstract In this study, the potent anti-tumor effects of brown algae on human leukemia HL-60 cells were investigated. The Sargassum siliquastrum extract among the 14 species of brown algae exhibited profound growth inhibitory effect on HL-60 cells in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, therefore, S. siliquastrum was selected for use in further experiments. The highest inhibitory activity of S. siliquastrum on HL-60 cells was detected in the chloroform fraction, and the active compound was identified as a kind of chromene, sargachromanol E (SE). SE treatment showed significant growth inhibitory effects on HL-60 cells in a dose-dependent manner by inducing apoptosis, as evidenced by the formation of apoptotic bodies, fragmented DNA ladder, and the accumulation of DNA in the sub-G1 phase of cell cycle. SE induced apoptosis was accompanied by downregulation of Bcl-xL, upregulation of Bax, activation of caspase-3, and cleavage of poly (ADP-ribose) polymerase (PARP). Moreover, z-DEVD-fmk, a caspase-3 inhibitor, significantly inhibited cell cytotoxicity, apoptotic characteristics such as apoptotic bodies, sub-G1 DNA content, and cleavage of PARP induced by SE. These results suggest that SE exerts its growth inhibitory effects on HL-60 cells through caspase-3-mediated induction of apoptosis. Therefore, SE offers promising chemotherapeuric potential to prevent c -
dc.description.uri 2 -
dc.language English -
dc.publisher 한국조류학회 -
dc.relation.isPartOf 제25회 한국조류학회 학술발표대회 -
dc.title Chromene induces apoptosis via caspase-3 activation in human leukemia HL-60 cells -
dc.type Conference -
dc.citation.conferencePlace KO -
dc.citation.endPage 108 -
dc.citation.startPage 108 -
dc.citation.title 제25회 한국조류학회 학술발표대회 -
dc.contributor.alternativeName 예보람 -
dc.contributor.alternativeName 김태호 -
dc.contributor.alternativeName 정주영 -
dc.contributor.alternativeName 전선미 -
dc.contributor.alternativeName 권영경 -
dc.contributor.alternativeName 장지이 -
dc.contributor.alternativeName Abu Affan -
dc.contributor.alternativeName 오철홍 -
dc.contributor.alternativeName 강도형 -
dc.contributor.alternativeName 허수진 -
dc.identifier.bibliographicCitation 제25회 한국조류학회 학술발표대회, pp.108 -
dc.description.journalClass 2 -
Appears in Collections:
Jeju Research Institute > Jeju Marine Research Center > 2. Conference Papers
Jeju Research Institute > Jeju Bio Research Center > 2. Conference Papers
Jeju Research Institute > Tropical & Subtropical Research Center > 2. Conference Papers
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse