Chromene induces apoptosis via caspase-3 activation in human leukemia HL-60 cells
DC Field | Value | Language |
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dc.contributor.author | 예보람 | - |
dc.contributor.author | 김태호 | - |
dc.contributor.author | 정주영 | - |
dc.contributor.author | 전선미 | - |
dc.contributor.author | 권영경 | - |
dc.contributor.author | 장지이 | - |
dc.contributor.author | Abu Affan | - |
dc.contributor.author | 오철홍 | - |
dc.contributor.author | 강도형 | - |
dc.contributor.author | Jung-Ho Hyun | - |
dc.contributor.author | 허수진 | - |
dc.date.accessioned | 2020-07-16T15:31:12Z | - |
dc.date.available | 2020-07-16T15:31:12Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2011-08-16 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/28244 | - |
dc.description.abstract | In this study, the potent anti-tumor effects of brown algae on human leukemia HL-60 cells were investigated. The Sargassum siliquastrum extract among the 14 species of brown algae exhibited profound growth inhibitory effect on HL-60 cells in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, therefore, S. siliquastrum was selected for use in further experiments. The highest inhibitory activity of S. siliquastrum on HL-60 cells was detected in the chloroform fraction, and the active compound was identified as a kind of chromene, sargachromanol E (SE). SE treatment showed significant growth inhibitory effects on HL-60 cells in a dose-dependent manner by inducing apoptosis, as evidenced by the formation of apoptotic bodies, fragmented DNA ladder, and the accumulation of DNA in the sub-G1 phase of cell cycle. SE induced apoptosis was accompanied by downregulation of Bcl-xL, upregulation of Bax, activation of caspase-3, and cleavage of poly (ADP-ribose) polymerase (PARP). Moreover, z-DEVD-fmk, a caspase-3 inhibitor, significantly inhibited cell cytotoxicity, apoptotic characteristics such as apoptotic bodies, sub-G1 DNA content, and cleavage of PARP induced by SE. These results suggest that SE exerts its growth inhibitory effects on HL-60 cells through caspase-3-mediated induction of apoptosis. Therefore, SE offers promising chemotherapeuric potential to prevent c | - |
dc.description.uri | 2 | - |
dc.language | English | - |
dc.publisher | 한국조류학회 | - |
dc.relation.isPartOf | 제25회 한국조류학회 학술발표대회 | - |
dc.title | Chromene induces apoptosis via caspase-3 activation in human leukemia HL-60 cells | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 108 | - |
dc.citation.startPage | 108 | - |
dc.citation.title | 제25회 한국조류학회 학술발표대회 | - |
dc.contributor.alternativeName | 예보람 | - |
dc.contributor.alternativeName | 김태호 | - |
dc.contributor.alternativeName | 정주영 | - |
dc.contributor.alternativeName | 전선미 | - |
dc.contributor.alternativeName | 권영경 | - |
dc.contributor.alternativeName | 장지이 | - |
dc.contributor.alternativeName | Abu Affan | - |
dc.contributor.alternativeName | 오철홍 | - |
dc.contributor.alternativeName | 강도형 | - |
dc.contributor.alternativeName | 허수진 | - |
dc.identifier.bibliographicCitation | 제25회 한국조류학회 학술발표대회, pp.108 | - |
dc.description.journalClass | 2 | - |