Transcrioptome Analysis of Light-utilizing Strain Candidatus Puniceispirillum marinum IMCC1322, a Member of SAR116 Group Bacteria

Abstract

The purpose of this study is to elucidate the proteorhodopsin function based on the genome of strain IMCC1322, a cultivated SAR116 group from East Sea, Korea. Light-enhanced photoheterotrophy of strain IMCC1322, whose phenotypic characteristics were revealed by cultivation and polyphasic studies, was interrogated by the transcriptomics. Samples of mRNA of dark/light/diel IMCC1322 cultures were obtained at days 3 and 6 post inoculation. The obtained sequence reads mRNAs were mapped to the IMCC1322 genome (GenBank Acc. No. CP001751). Here we report the mRNA profiling of IMCC1322 which might be the first attempt to the biogeochemistry of light-utilizing SAR116 group in the ocean and the transcriptomes revealed that green light (max. wavelength =522nm) drives respiratory metabolism and cessation of bacterial aging after stationary phase.