Transcriptomic responses in Benzo[a]pyrene exposed Hydra

Abstract

To define the repertoire of Hydra genes responding to acute exposure of benzo[a]pyrene (BaP), transcriptome dynamics were examined in a series of exposure time (4 h, 12 h, 24 h, and 48 h) by using DNA microarray which contain about 18,000 singletons. During the exposure at nonlethal concentration of BaP (500 μg/L, 1/50 48 h LC50), the drastic changes in transcript level were found between 12 and 24 h. Genes induced or repressed in the early stage of exposure (4 h) included in molecular chaperon, anti-oxidant enzyme, apoptosis, necrosis and cancer related cellular processes. The levels of transcripts which related to development, inflammation, calcium metabolism, Na-K channel, innate immune were affected at 12 h exposure. At the 24 h exposure, the expression level of genes involved in sexual development, neurotransmission, and tumor process were found to be altered. Finally, the expression level of DNA repair, ubiquitination, development, differentiation, tumor and cancer related genes were found to be affected at 48 h exposure. Taken together, the results obtained in differential gene expression profiles at various exposure time offer valuable transcriptomic resources for understanding the biological responses to BaP toxicity.00 singletons. During the exposure at nonlethal concentration of BaP (500 μg/L, 1/50 48 h LC50), the drastic changes in transcript level were found between 12 and 24 h. Genes induced or repressed in the early stage of exposure (4 h) included in molecular chaperon, anti-oxidant enzyme, apoptosis, necrosis and cancer related cellular processes. The levels of transcripts which related to development, inflammation, calcium metabolism, Na-K channel, innate immune were affected at 12 h exposure. At the 24 h exposure, the expression level of genes involved in sexual development, neurotransmission, and tumor process were found to be altered. Finally, the expression level of DNA repair, ubiquitination, develop