Evaluating Carriers for Immobilizing Saccharomyces cerevisiae for Ethanol Production in a Continuous Column Reactor SCIE SCOPUS KCI

Cited 1 time in WEB OF SCIENCE Cited 1 time in Scopus
Title
Evaluating Carriers for Immobilizing Saccharomyces cerevisiae for Ethanol Production in a Continuous Column Reactor
Author(s)
Cha, Hye-Geun; Kim, Yi-Ok; Choi, Woon Yong; Kang, Do-Hyung; Lee, Hyeon-Yong; Jung, Kyung-Hwan
KIOST Author(s)
Kang, Do Hyung(강도형)
Publication Year
2014-09
Abstract
We evaluated a more practical and cost-effective immobilization carriers for ethanol production using the yeast Saccharomyces cerevisiae. Three candidate materials-rice hull, rice straw, and sawdust-were tested for their cell-adsorption capacity and operational durability. Derivatizations of rice hull, rice straw, and sawdust with the optimal concentration of 0.5 M of 2-(diethylamino) ethyl chloride hydrochloride (DEAE . HCl) resulted in > 95% adsorption of the initial yeast cells at 2 hr for DEAE-rice hull and DEAE-sawdust and in only approximately 80% adsorption for DEAE-rice straw. In addition, DEAE-sawdust was found to be a more practical carrier for immobilizing yeast cells in terms of operational durability in shaking flask cultures with two different speeds of 60 and 150 rpm. Furthermore, the biosorption isotherms of DEAE-rice hull, -rice straw, and -sawdust for yeast cells revealed that the Q(max) of DEAE-sawdust (82.6 mg/g) was greater than that of DEAE-rice hull and DEAE-rice straw. During the 404-hr of continuous column reactor operation using yeast cells immobilized on DEAE-sawdust, no serious detachment of the yeast cells from the DEAE-sawdust was recorded. Ethanol yield of approximately 3.04 g/L was produced steadily, and glucose was completely converted to ethanol at a yield of 0.375 g-ethanol/g-glucose (73.4% of the theoretical value). Thus, sawdust is a promising practical immobilization carrier for ethanol production, with significance in the production of bioethanol as a biofuel.
ISSN
1229-8093
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2734
DOI
10.5941/MYCO.2014.42.3.249
Bibliographic Citation
MYCOBIOLOGY, v.42, no.3, pp.249 - 255, 2014
Publisher
KOREAN SOC MYCOLOGY
Subject
SURFACE-AERATED FERMENTER; REPEATED-BATCH OPERATION; ALCOHOLIC FERMENTATION; BIOETHANOL PRODUCTION; SUPPORT MATERIALS; WINE-MAKING; YEAST; HYDROLYSATE; BAGASSE; CELLS
Keywords
Continuous column reactor; Ethanol production; Immobilization carrier; Saccharomyces cerevisiae; Sawdust
Type
Article
Language
English
Document Type
Article
Publisher
KOREAN SOC MYCOLOGY
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