불규칙적인 세포 주기의 세포 분열능 측정

Abstract

To obtain a better understanding of the bloom development mechanism in the aquatic systems, accurate estimates of species-specific in situ growth rates are needed for the migration and bloom mechanisms of red tide. The harmful algal blooms caused by fast cell division of microorganisms. To estimate accurate in situ growth rate of H. akashiwo which has non-uniform and irregular cell cycles, we modified equation based on the cell cycle and calculated the in situ growth rate to describe their bloom developmental process in nature. Sampling was conducted every three hours from 15:00 on August 2 to 7:00 on August 4, 2006 in Pohang Bay, Korea. DNA amounts in the H. akashiwo were measured with a flow cytometer following tyramide signal amplification-fluorescence in situ hybridization (TSA-FISH). During the first night, the percentage of G1 phase cells decreased from 15:00 to 19:00 and increased until 22:00. It dramatically decreased from 22:00 on 2 August and increased from 7:00 to10:00 on 3 August. These results suggest the capability of dividing more than once d-1. Similar results were obtained the following night but division did not occur twice during the second night. Based on the results, in situ growth rates ranged from 0.31 to 0.53 d-1. We conclude that, with the aid of this newly derived equation, the blooming formation by fast cell division could estimate more accurate.oms caused by fast cell division of microorganisms. To estimate accurate in situ growth rate of H. akashiwo which has non-uniform and irregular cell cycles, we modified equation based on the cell cycle and calculated the in situ growth rate to describe their bloom developmental process in nature. Sampling was conducted every three hours from 15:00 on August 2 to 7:00 on August 4, 2006 in Pohang Bay, Korea. DNA amounts in the H. akashiwo were measured with a flow cytometer following tyramide signal amplification-fluorescence in situ hybridization (TSA-FISH). D