One-step sequence- and ligation-independent cloning (SLIC): Rapid and versatile cloning method for functional genomics studies

Title
One-step sequence- and ligation-independent cloning (SLIC): Rapid and versatile cloning method for functional genomics studies
Author(s)
강성균; 정재연; 임형순; 류지영; 이현숙; 신동승; 이정현
KIOST Author(s)
Kang, Sung Gyun(강성균)Yim, Hyung Soon(임형순)Lee, Hyun Sook(이현숙)Lee, Jung Hyun(이정현)
Publication Year
2013-05-01
Abstract
The need for high-throughput recombinant DNA technology is demanding due to the rapidly increased interest in functional genomics studies following the surge of data generated by next generation sequencing. We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. One-step SLIC offer a uniform conversion of genes into one context to another and would make high-throughput gene cloning practical for functional genomics studies and greatly facilitate synthetic biology. This work was supported by KORDI in-house program, the MEGRC program and the Development of Biohydrogen Production Technology using the Hyperthermophilic Archaea program of the Ministry of Land, Transport, and Maritime Affairs.e- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. One-step SLIC offer a uniform conversion of genes into one context to another and would make high-throughput gene cloning practical for functional genomics studies and greatly facilitate synthetic biology.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/27102
Bibliographic Citation
International Meeting of the Microbiological Society of Korea, pp.285, 2013
Publisher
한국미생물학회
Type
Conference
Language
English
Publisher
한국미생물학회
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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