One-step sequence- and ligation-independent cloning (SLIC): Rapid and versatile cloning method for functional genomics studies
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Title
- One-step sequence- and ligation-independent cloning (SLIC): Rapid and versatile cloning method for functional genomics studies
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Author(s)
- 강성균; 정재연; 임형순; 류지영; 이현숙; 신동승; 이정현
- KIOST Author(s)
- Kang, Sung Gyun(강성균); Yim, Hyung Soon(임형순); Lee, Hyun Sook(이현숙); Lee, Jung Hyun(이정현)
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Alternative Author(s)
- 강성균; 정재연; 임형순; 류지영; 이현숙; 이정현
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Publication Year
- 2013-05-01
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Abstract
- The need for high-throughput recombinant DNA technology is demanding due to the rapidly increased interest in functional genomics studies following the surge of data generated by next generation sequencing. We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. One-step SLIC offer a uniform conversion of genes into one context to another and would make high-throughput gene cloning practical for functional genomics studies and greatly facilitate synthetic biology. This work was supported by KORDI in-house program, the MEGRC program and the Development of Biohydrogen Production Technology using the Hyperthermophilic Archaea program of the Ministry of Land, Transport, and Maritime Affairs.e- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. One-step SLIC offer a uniform conversion of genes into one context to another and would make high-throughput gene cloning practical for functional genomics studies and greatly facilitate synthetic biology.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/27102
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Bibliographic Citation
- International Meeting of the Microbiological Society of Korea, pp.285, 2013
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Publisher
- 한국미생물학회
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Type
- Conference
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Language
- English
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