DIFFERENT TOXIC SENSITIVITY BETWEEN EMBRYONIC FLOUNDER (PARALICHTHYS OLIVACEUS) AND SPOTTED SEA BASS (LATEOLABRAX MACULATES) EXPOSED TO IRANIAN HEAVY CRUDE OIL MALFORMATION AND CYP1A EXPRESSION

Title
DIFFERENT TOXIC SENSITIVITY BETWEEN EMBRYONIC FLOUNDER (PARALICHTHYS OLIVACEUS) AND SPOTTED SEA BASS (LATEOLABRAX MACULATES) EXPOSED TO IRANIAN HEAVY CRUDE OIL MALFORMATION AND CYP1A EXPRESSION
Author(s)
정지현; 채영선; 김하나; 심원준; J.P.Incardona
KIOST Author(s)
Jung, Jee Hyun(정지현)Shim, Won Joon(심원준)
Publication Year
2013-05-13
Abstract
M/V Hebei sprit oil spill (HSOS) which is considered as a one of the largest tanker spills of recent years, comparable to the Prestige oil spill in 2002 and Tasman Spirit in 2003. In the study, to clarify the effects of spilled oil on resident fish development, spotted bass (Lateolabrax maculates) and olive flounder (Paralichthys olivaceus) embryos (48 hr post-fertilization) were exposed to oiled gravel effluent of Iranian Heavy crude oil (IHCO) oil for 48 hr. Embryos were manually dechorionated and observed by a stereomicroscope and a compound microscope. Embryos were fixed in 4% phosphate-buffered paraformaldehyde and stained for CYP1A and myosin heavy chain. After 24 hr, several complex effects, including the pericardium edema and tail mutant were observed in both of embryos after the IHCO exposure. The distribution of CYP1A in embryo was assessed by immunofluorescence. CYP1A immunofluorescence activity was high significantly in the endothelium of head, epidermis and cardium and a significant induction of CYP1A mRNA was observed in both of embryos after the oil exposure. The extent of tail abnormalities and edema in olive flounder were stronger than those of spotted bass in oil exposed groups. But the levels of CYP1A expression were lower than those of spotted bass. Our results confirm that the HSOS caused significant damage to resident fish embryos and different toxic sensitivity weresident fish development, spotted bass (Lateolabrax maculates) and olive flounder (Paralichthys olivaceus) embryos (48 hr post-fertilization) were exposed to oiled gravel effluent of Iranian Heavy crude oil (IHCO) oil for 48 hr. Embryos were manually dechorionated and observed by a stereomicroscope and a compound microscope. Embryos were fixed in 4% phosphate-buffered paraformaldehyde and stained for CYP1A and myosin heavy chain. After 24 hr, several complex effects, including the pericardium edema and tail mutant were observed in both of embryos after the IHCO exposure. The distribution of CYP1A in embryo was assessed by immunofluorescence. CYP1A immunofluorescence activity was high significantly in the endothelium of head, epidermis and cardium and a significant induction of CYP1A mRNA was observed in both of embryos after the oil exposure. The extent of tail abnormalities and edema in olive flounder were stronger than those of spotted bass in oil exposed groups. But the levels of CYP1A expression were lower than those of spotted bass.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/27069
Bibliographic Citation
17th Pollutant Responses in Marine Organisms, pp.280, 2013
Publisher
17th PRIMO
Type
Conference
Language
English
Publisher
17th PRIMO
Related Researcher
Research Interests

Microplastic pollution,Persistent Organic Pollutants,Oil Pollution,미세플라스틱 오염,잔류성 유기오염물질,유류오염

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