Identification of hypoxia biomarker candidates from sea urchin (Strongylocentrotus nudus) coelomocyte by cDNA microarray

Title
Identification of hypoxia biomarker candidates from sea urchin (Strongylocentrotus nudus) coelomocyte by cDNA microarray
Author(s)
이건섭; 황진익; 박미례; 이택견
KIOST Author(s)
Lee, Taek Kyun(이택견)
Alternative Author(s)
이건섭; 황진익; 박미례; 이택견
Publication Year
2013-05-24
Abstract
Dissolved oxygen is one of the most important factors controlling growth in aquatic organisms. Hypoxia is generally defined as dissolved oxygen less than 2.8 mg O2/L(equivalent to 2 mL O2/L or 91.4 mM). In this study, increasing of red spherule cell and gene expression change by microarray analysis in sea urchin (Strongylocentrotus nudus) coelomocytes was accomplished to identify cellular stress of hypoxia condition. Adult sea urchins were exposed to dissolved oxygen levels of 7.6 mg O2/L (normal), 4.7 mg O2/L and 1.6 mg O2/L (hypoxia), at 15°C and 33‰. The each coelomocytes of Strongylocentrotus nudus were harvested at 0 hr, 6 hr, 12 hr and 24 hr. A significant increasing of the red spherule cells was observed hypoxic condition sample at 24 hr. To process molecular work, microarray analysis was conducted. Several genes in hypoxia group (24 hr) were significantly reduced than control group (normal, 0 hr). These genes (RNA processing and modification related genes: RNA helicase and pyrophosphatase DCP2, transcription related genes: CDP1 and GATA-4/5/6, cell cycle control related gene: spindle pole body, defence mechanism related gene: C-type lectin) were identified by qReal-time PCR. We identified that gene expression of hypoxia group was reduced by up to 20%-80% compared to control group at 24 hr. RNA helicase and GATA-4/5/6 were reduced by 60% and 80% in the field samples, respectively. Taken all together, our resrule cell and gene expression change by microarray analysis in sea urchin (Strongylocentrotus nudus) coelomocytes was accomplished to identify cellular stress of hypoxia condition. Adult sea urchins were exposed to dissolved oxygen levels of 7.6 mg O2/L (normal), 4.7 mg O2/L and 1.6 mg O2/L (hypoxia), at 15°C and 33‰. The each coelomocytes of Strongylocentrotus nudus were harvested at 0 hr, 6 hr, 12 hr and 24 hr. A significant increasing of the red spherule cells was observed hypoxic condition sample at 24 hr. To process molecular work, microarray analysis was conducted. Several genes in hypoxia group (24 hr) were significantly reduced than control group (normal, 0 hr). These genes (RNA processing and modification related genes: RNA helicase and pyrophosphatase DCP2, transcription related genes: CDP1 and GATA-4/5/6, cell cycle control related gene: spindle pole body, defence mechanism related gene: C-type lectin) were identified by qReal-time PCR. We identified that gene expression of hypoxia group was reduced by up to 20%-80% compared to control group at 24 hr. RNA helicase and GATA-4/5/6 were reduced by 60% and 80% in the field samples, respectively. Taken all together, our res
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/26983
Bibliographic Citation
2013 한국해양학회, pp.421, 2013
Publisher
한국해양과학기술협의회
Type
Conference
Language
English
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