The Primary Toxic Effects on Marine Embryonic Fish from Oil Exposure Different Toxic Sensitivity between Flounder (Paralichthys Olivaceus) and Spotted Sea Bass (Lateolabrax Maculates)

Abstract

In the study, to determine (1) if novel phenotypes were associatedwith exposure to IHCO, (2) whether the effects of IHCO on heartdevelopment and tail fin defect (3) whether the different fish speciesmight have different specific patterns of CYP1A induction, spottedbass (Lateolabrax maculates) and olive flounder (Paralichthysolivaceus) embryos (48 hr post-fertilization) were exposed to oiledgravel effluent of Iranian Heavy crude oil (IHC) oil for 48 hr. After24 hr, several complex effects, including the pericardium edema andtail fin mutant were observed in both of embryos after the IHCexposure. The distribution of CYP1A in embryo was assessed byimmunofluorescence. CYP1A immunofluorescence activity was highsignificantly in the endothelium of head, epidermis and pericardium.Significant induction of CYP1A mRNA was observed in bothof embryos after the oil exposure. The extent of tail abnormalitiesand edema in olive flounder were stronger than those of spotted bassin oil exposed groups. But the levels of CYP1A expression werelower than those of spotted bass. Our results confirm that the HSOScaused significant damage to resident fish embryos and differenttoxic sensitivity were investigated in both species at morphologicaland biochemical levels.rns of CYP1A induction, spottedbass (Lateolabrax maculates) and olive flounder (Paralichthysolivaceus) embryos (48 hr post-fertilization) were exposed to oiledgravel effluent of Iranian Heavy crude oil (IHC) oil for 48 hr. After24 hr, several complex effects, including the pericardium edema andtail fin mutant were observed in both of embryos after the IHCexposure. The distribution of CYP1A in embryo was assessed byimmunofluorescence. CYP1A immunofluorescence activity was highsignificantly in the endothelium of head, epidermis and pericardium.Significant induction of CYP1A mRNA was observed in bothof embryos after the oil exposure. The extent of tail abnormalitiesand edema in olive flounder were stronger than those of spotted bassin oil exposed groups. But the levels of CYP1A expression werelower than those of spotted bass. Our results confirm that the HSOScaused significant damage to resident fish embryos and differenttoxic sensitivity were investigated in both species at morphologicaland biochemical levels.