Inhibition of tumor growth in vitro and in vivo by fucoxanthin against human leukemia HL-60 cells and melanoma B16F10 cells
DC Field | Value | Language |
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dc.contributor.author | 허수진 | - |
dc.contributor.author | 예보람 | - |
dc.contributor.author | 장지이 | - |
dc.contributor.author | 김준성 | - |
dc.contributor.author | 김민선 | - |
dc.contributor.author | 김지형 | - |
dc.contributor.author | 이영득 | - |
dc.contributor.author | 이수진 | - |
dc.contributor.author | 오철홍 | - |
dc.contributor.author | 강도형 | - |
dc.date.accessioned | 2020-07-16T08:31:17Z | - |
dc.date.available | 2020-07-16T08:31:17Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2013-07-10 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/26867 | - |
dc.description.abstract | In the present study, we designed to evaluate the molecular mechanism of fucoxanthin isolated from brown algae against HL-60 and B16F10 cell lines. We found that ROS are generated during fucoxanthin-induced apoptosis in HL-60 cells, and NAC which is a scavenger of ROS, suppressed fucoxantin-induced cytotoxicity and apoptosis. Furthermore, the treatment with NAC dramatically inhibited fucoxanthin-induced phosphorylation of JNK and p38 kinase in HL-60 cells. Moreover, fucoxanthin reduced the viability of B16F10 cells in a dose-dependent manner accompanied by the induction of cell cycle arrest during the G0/G1 phase and apoptosis. Fucoxanthin-induced G0/G1 arrest was associated with a marked decrease in the protein expressions of phosphorylated-Rb, cyclin D and Cdk4 and up-regulation of the protein levels of p15INK4B and p27Kip1. Fucoxanthin-induced apoptosis was accompanied with down-regulation of the protein levels of Bcl-xL, and inhibitor of apoptosis protein, resulting in cytochrome c release and sequential activation of caspase-9, caspase-3, and PARP. Furthermore, fucoxanthin activated JNK, MAPK and ERK on B16F10 cells. These results suggest that fucoxanthin has anti-tumor effects on HL-60 and B16F10 cell lines by inducing selectively the genes related to apoptosis which provided further theoretical support for the application of fucoxanthin as a promising anti-tumor agent. which is a scavenger of ROS, suppressed fucoxantin-induced cytotoxicity and apoptosis. Furthermore, the treatment with NAC dramatically inhibited fucoxanthin-induced phosphorylation of JNK and p38 kinase in HL-60 cells. Moreover, fucoxanthin reduced the viability of B16F10 cells in a dose-dependent manner accompanied by the induction of cell cycle arrest during the G0/G1 phase and apoptosis. Fucoxanthin-induced G0/G1 arrest was associated with a marked decrease in the protein expressions of phosphorylated-Rb, cyclin D and Cdk4 and up-regulation of the protein levels of p15INK4B and p27Kip1. Fucoxanthin-induced apoptosis was accompanied with down-regulation of the protein levels of Bcl-xL, and inhibitor of apoptosis protein, resulting in cytochrome c release and sequential activation of caspase-9, caspase-3, and PARP. Furthermore, fucoxanthin activated JNK, MAPK and ERK on B16F10 cells. These results suggest that fucoxanthin has anti-tumor effects on HL-60 and B16F10 cell lines by inducing selectively the genes related to apoptosis which provided further theoretical support for the application of fucoxanthin as a promising anti-tumor agent. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | 한국응용생명화학회 | - |
dc.relation.isPartOf | 2013 International Symposium and Annual Meeting of the KSABC | - |
dc.title | Inhibition of tumor growth in vitro and in vivo by fucoxanthin against human leukemia HL-60 cells and melanoma B16F10 cells | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 206 | - |
dc.citation.startPage | 206 | - |
dc.citation.title | 2013 International Symposium and Annual Meeting of the KSABC | - |
dc.contributor.alternativeName | 허수진 | - |
dc.contributor.alternativeName | 예보람 | - |
dc.contributor.alternativeName | 장지이 | - |
dc.contributor.alternativeName | 김준성 | - |
dc.contributor.alternativeName | 김민선 | - |
dc.contributor.alternativeName | 김지형 | - |
dc.contributor.alternativeName | 이영득 | - |
dc.contributor.alternativeName | 이수진 | - |
dc.contributor.alternativeName | 오철홍 | - |
dc.contributor.alternativeName | 강도형 | - |
dc.identifier.bibliographicCitation | 2013 International Symposium and Annual Meeting of the KSABC, pp.206 | - |
dc.description.journalClass | 1 | - |