Evaluation of sediment pollution in three local waste dumping sites using polychaeta, Perinereis nuntia

Abstract

This study aimed to examine the biological effects of marine sediment layer in dumping sites using the sediment-dwelling organism at transcription level. The marine sediments were collected from 6 pumping sites, 1 non-dumping site (YBR-001) and 5 dumping sites (YB-009, YB-049, YB-053, YB-098, YB-101) in the Yellow Sea 5 pumping sites, 1 non-dumping site (DBR-010) and 4 dumping sites (DB-026, DB-079, DB-089, DB-091) in the East Sea-B sites 5 pumping , 1 non-dumping site (DJR-009) and 4 dumping sites(DJ-016, DJ-056, DJ-059, DJ-064) in the East Sea-J sites. The pore waters were prepared from each sediment sample. The polychaetes were incubated in the pore waters and the expression level of 9 target genes were compared with those of a group exposed to control seawater and the a group exposed to 200 ppb of CdCl2 for 24 hours, respectively. The target genes are 2 metal binding genes (ferritin and hemerythrin-like metalloprotein) and 7 oxidative stress related genes (catalase, glutathione peroxidase 1, glutathione peroxidase 3, glutathione S-transferase-α, heat shock protein 70, heat shock protein 90α and Mn superoxide dismutase). Considering the range of gene expression level changes, the degree of sediment pollution could be ranked as YB-053 > 101 > 098 ≥ 049 > YBR-001 in the Yellow Sea DB-026 > 092 > 079 > 089 in the East Sea-B sites DJ-056 ≥ 059 > 016 > 064 in the East Sea-J sites. and 5 dumping sites (YB-009, YB-049, YB-053, YB-098, YB-101) in the Yellow Sea 5 pumping sites, 1 non-dumping site (DBR-010) and 4 dumping sites (DB-026, DB-079, DB-089, DB-091) in the East Sea-B sites 5 pumping , 1 non-dumping site (DJR-009) and 4 dumping sites(DJ-016, DJ-056, DJ-059, DJ-064) in the East Sea-J sites. The pore waters were prepared from each sediment sample. The polychaetes were incubated in the pore waters and the expression level of 9 target genes were compared with those of a group exposed to control seawater and the a group exposed to 200 ppb of CdCl2 for 24 hours, respectively. The target genes are 2 metal binding genes (ferritin and hemerythrin-like metalloprotein) and 7 oxidative stress related genes (catalase, glutathione peroxidase 1, glutathione peroxidase 3, glutathione S-transferase-α, heat shock protein 70, heat shock protein 90α and Mn superoxide dismutase). Considering the range of gene expression level changes, the degree of sediment pollution could be ranked as YB-053 > 101 > 098 ≥ 049 > YBR-001 in the Yellow Sea DB-026 > 092 > 079 > 089 in the East Sea-B sites DJ-056 ≥ 059 > 016 > 064 in the East Sea-J sites.