Anti-osteoclastogenic Effect and Action Mechanisms of the Chromene Isolated from Brown Seaweed

Title
Anti-osteoclastogenic Effect and Action Mechanisms of the Chromene Isolated from Brown Seaweed
Author(s)
허수진; 윤원종; 예보람; 김민선; 장지이; 오철홍; 강도형
KIOST Author(s)
Heo, Soo Jin(허수진)Oh, Chulhong(오철홍)Kang, Do-Hyung(강도형)
Publication Year
2013-10-17
Abstract
In this present study, we investigated the anti-osteoclastogenic effects of sargachromanol G, a kind of chromene, isolated from Sargassum silquastrum on the expressin of IL-1β-induced osteoclastogenic factors (RANKL, IL-6, PGE2, and COX-2) in human osteoblast MG-63 cells, as well as LPS or RANKL-induced pro-inflammatory factors and osteoclastogenic factor [nitric oxide (NO), cytokines (TNF-α, IL-1β, and IL-6), TRAP, CTR, TRAF6, Cath-K, and MMP-9] in murine macrophage RAW 264.7 cells. We also examined the role of nuclear factor- κB (NF-κB) and mitogen activated protein kinase (MAPK) signaling induced by IL-1β in MG-63 and LPS or RANKL in RAW 264.7 cells. Sargachromanol G dose-dependently inhibited the production of osteoclastogenic factors in MG-63 and RAW 264.7 cells. Sargachromanol G also inhibited phosphorylation of NF-κB (IκB- α, p65, and p50) and MAPK (ERK1/2, JNK, and p38). These results suggest that the anti-osteoclastogenic activity of sargachromanol G may result from modulation of osteoclastogenic factors and cytokines via suppression of phosphorylated MAPK and NF-κB activation. in human osteoblast MG-63 cells, as well as LPS or RANKL-induced pro-inflammatory factors and osteoclastogenic factor [nitric oxide (NO), cytokines (TNF-α, IL-1β, and IL-6), TRAP, CTR, TRAF6, Cath-K, and MMP-9] in murine macrophage RAW 264.7 cells. We also examined the role of nuclear factor- κB (NF-κB) and mitogen activated protein kinase (MAPK) signaling induced by IL-1β in MG-63 and LPS or RANKL in RAW 264.7 cells. Sargachromanol G dose-dependently inhibited the production of osteoclastogenic factors in MG-63 and RAW 264.7 cells. Sargachromanol G also inhibited phosphorylation of NF-κB (IκB- α, p65, and p50) and MAPK (ERK1/2, JNK, and p38). These results suggest that the anti-osteoclastogenic activity of sargachromanol G may result from modulation of osteoclastogenic factors and cytokines via suppression of phosphorylated MAPK and NF-κB activation.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/26689
Bibliographic Citation
2013 한국생물공학회 추계학술발표대회 및 국제심포지엄, pp.71, 2013
Publisher
한국생물공학회
Type
Conference
Language
English
Publisher
한국생물공학회
Related Researcher
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse