Biomass Evaluation of a Novel Green Microalga Chlamydomonas sp. KIOST-1 for Biofuel Production Isolated from Korea

Title
Biomass Evaluation of a Novel Green Microalga Chlamydomonas sp. KIOST-1 for Biofuel Production Isolated from Korea
Author(s)
전선미; 김지형; 박아름이; 김태호; 이수진; 오철홍; 허수진; 강도형
KIOST Author(s)
Park, Areumi(박아름이)Kim, Tae Ho(김태호)Oh, Chul Hong(오철홍)Heo, Soo Jin(허수진)Kang, Do Hyung(강도형)
Alternative Author(s)
전선미; 김지형; 박아름이; 김태호; 이수진; 오철홍; 허수진; 강도형
Publication Year
2013-11-11
Abstract
From the early 20th century, Chlamydomonas (Chlorophyceae, Chlorophyta) has been thoroughly investigated as an important model for microalgal physiology and genetics. Recently, those researches also have been expanded on bioremediation and biofuel production. In this study, we evaluated the biofuel production potential of a newly isolated unicellular green microalga from freshwater in Korea. The isolated microalga was preliminarily analyzed by microscopic investigations and its taxonomical position was confirmed by two phylogenetic analysis using 18S rRNA and ITS1-5.8S-ITS2 sequences, respectively. Based on these results, the isolated microalga belongs to the genus Chlamydomonas, and finally designated as Chlamydomonas sp. KIOST-1. In order to evaluate its potential for biofuel production, cellular components (protein, lipid, carbohydrate, ash and moisture) of the isolate were analyzed. According to the biochemical analysis of Chlamydomonas sp. KIOST-1, its protein, lipid, carbohydrate, ash and moisture contents were estimated to 53.5 ± 0.8%, 20.6 ± 0.8%, 16.9 ± 1.1%, 5.2 ± 1.2% and 3.9 ± 0.1%, respectively. Furthermore, protein and carbohydrate of the microalga were mainly comprised of glutamic-acid (11.6%) and D-glucose (49.7%), respectively. Interestingly, oleic acid (33.1 ± 0.1%) known as important sources for biodiesel production, were dominantly found from Chlamydomonas sp. KIOST-1. Based on these resultsbiofuel production. In this study, we evaluated the biofuel production potential of a newly isolated unicellular green microalga from freshwater in Korea. The isolated microalga was preliminarily analyzed by microscopic investigations and its taxonomical position was confirmed by two phylogenetic analysis using 18S rRNA and ITS1-5.8S-ITS2 sequences, respectively. Based on these results, the isolated microalga belongs to the genus Chlamydomonas, and finally designated as Chlamydomonas sp. KIOST-1. In order to evaluate its potential for biofuel production, cellular components (protein, lipid, carbohydrate, ash and moisture) of the isolate were analyzed. According to the biochemical analysis of Chlamydomonas sp. KIOST-1, its protein, lipid, carbohydrate, ash and moisture contents were estimated to 53.5 ± 0.8%, 20.6 ± 0.8%, 16.9 ± 1.1%, 5.2 ± 1.2% and 3.9 ± 0.1%, respectively. Furthermore, protein and carbohydrate of the microalga were mainly comprised of glutamic-acid (11.6%) and D-glucose (49.7%), respectively. Interestingly, oleic acid (33.1 ± 0.1%) known as important sources for biodiesel production, were dominantly found from Chlamydomonas sp. KIOST-1. Based on these results
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/26561
Bibliographic Citation
The International Marine Biotechnology Conference 2013, pp.100, 2013
Publisher
IMBCs
Type
Conference
Language
English
Publisher
IMBCs
Related Researcher
Research Interests

Microalgae Culture and Application,Aquaculture System Engineering,미세조류 배양 및 응용,배양시스템 엔지니어링,유용해양생물 배양 및 응용

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