Comparative analysis of biochemical components in Spirulina maxima Cy-23 and the newly isolated Leptolyngbya sp. KIOST-1

Abstract

Numerous approaches have been attempted to produce microalgal biomass for commercial exploitations. In recent years, Leptolyngbya sp. KIOST-1 was isolated from the Spirulina culture raceway in Korea. In order to evaluate its potential for industrial applications, the compositions of cellular components including amino acids, mono-saccharide and fatty acids (FAs) in the isolate were investigated and compared to those in Spirulina maxima Cy-23 strain which was cultured at the raceway. From the Leptolyngbya strain, ash, carbohydrate, lipid and moisture contents were estimated to be 10.2±0.1%, 24.5±0.7%, 11.4±0.5% and 1.3±0.1%, respectively. And its protein content was estimated to be 52.6±0.1%, thus proving that it is major component in the isolate. Similarly, protein (50.7±0.3%) was dominant cellular component in Spirulina strain, and its ash, carbohydrate, lipid and moisture contents were estimated to be 20.3±0.3%, 17.8±0.3%, 9.1±0.5% and 2.2±0.1%, respectively. Moreover, glutamic-acid (14.2% and 14.7%) and aspartic-acid (11.0% and 10.2%) were identified as dominant amino acids from the Leptolyngbya and Spirulina strains, and D-glucose (75.9% and 80.3%) was dominant mono-saccharide in those two cyanobacteria. In the FAs analysis, unsaturated FAs such as 16:1 w9c (18.5%), 18:1 w9c (21.4%) and 18:2 w6,9c/18:0 ANTE (16.9%), and 16:0 saturated FAs (17.3%) were dominantly found from the isolate. However, the Spirndustrial applications, the compositions of cellular components including amino acids, mono-saccharide and fatty acids (FAs) in the isolate were investigated and compared to those in Spirulina maxima Cy-23 strain which was cultured at the raceway. From the Leptolyngbya strain, ash, carbohydrate, lipid and moisture contents were estimated to be 10.2±0.1%, 24.5±0.7%, 11.4±0.5% and 1.3±0.1%, respectively. And its protein content was estimated to be 52.6±0.1%, thus proving that it is major component in the isolate. Similarly, protein (50.7±0.3%) was dominant cellular component in Spirulina strain, and its ash, carbohydrate, lipid and moisture contents were estimated to be 20.3±0.3%, 17.8±0.3%, 9.1±0.5% and 2.2±0.1%, respectively. Moreover, glutamic-acid (14.2% and 14.7%) and aspartic-acid (11.0% and 10.2%) were identified as dominant amino acids from the Leptolyngbya and Spirulina strains, and D-glucose (75.9% and 80.3%) was dominant mono-saccharide in those two cyanobacteria. In the FAs analysis, unsaturated FAs such as 16:1 w9c (18.5%), 18:1 w9c (21.4%) and 18:2 w6,9c/18:0 ANTE (16.9%), and 16:0 saturated FAs (17.3%) were dominantly found from the isolate. However, the Spir